ArticleGenome research1999
A genetic strategy to eliminate self-activator baits prior to high-throughput yeast two-hybrid screens.
Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- The Intricacy of the Viral-Human Protein Interaction Networks: Resources, Data, and Analyses.Frontiers in microbiology · 2022Review
- The HTLV-1 viral oncoproteins Tax and HBZ reprogram the cellular mRNA splicing landscape.PLoS pathogens · 2021Article
- Screening of Duck Tembusu Virus NS3 Interacting Host Proteins and Identification of Its Specific Interplay Domains.Viruses · 2019Article
- Base-resolution stratification of cancer mutations using functional variomics.Nature protocols · 2017Article
- Proteome-Scale Human Interactomics.Trends in biochemical sciences · 2017Review
- A combined binary interaction and phenotypic map of C. elegans cell polarity proteins.Nature cell biology · 2016Article
- A Modified Reverse One-Hybrid Screen Identifies Transcriptional Activation Domains in PHYTOCHROME-INTERACTING FACTOR 3.Frontiers in plant science · 2016Article
- The yeast two-hybrid assay: still finding connections after 25 years.Nature methods · 2014Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Gene-centered yeast one-hybrid assays.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Next-generation sequencing to generate interactome datasets.Nature methods · 2011Article
- Gene-centered regulatory network mapping.Methods in cell biology · 2011Review
- High-quality binary protein interaction map of the yeast interactome network.Science (New York, N.Y.) · 2008Article
- Article
- Coverage and error models of protein-protein interaction data by directed graph analysis.Genome biology · 2007Article
- A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors.Nature biotechnology · 2005Article
- Effect of sampling on topology predictions of protein-protein interaction networks.Nature biotechnology · 2005Article
- Identification of critical domains and putative partners for the Caenorhabditis elegans spindle component LIN-5.Molecular genetics and genomics : MGG · 2004Article
- A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating.Genome research · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Large-scale sequencing projects have predicted high numbers of gene products for which no functional information is yet available. Hence, large-scale projects, such as gene knockouts, gene expression profiles, and protein-interaction mapping, are currently under way to initiate the understanding of the function of these gene products. The high-throughput strategies that are currently being developed to generate protein-interaction maps include automated versions of the yeast two-hybrid system. These strategies rely on the large-scale construction of DNA-binding domain/protein-of-interest hybrid constructs (DB-X baits). An inherent problem of large-scale two-hybrid systems is that a high percentage of cloned sequences encode polypeptides that, when fused to DB, can activate transcription in the absence of any two-hybrid-interacting partner protein. Here, we describe and validate a genetic strategy that efficiently eliminates such self-activator baits prior to screening procedures. The strategy is based on a negative-growth selection and is compatible with high-throughput settings.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.