ArticleMolecular and cellular biology1999
Analysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Mechanisms and Functions of the RNA Polymerase II General Transcription Machinery during the Transcription Cycle.Biomolecules · 2024Review
- Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1.Current genetics · 2023Article
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- Article
- Direct TFIIA-TFIID protein contacts drive budding yeast ribosomal protein gene transcription.The Journal of biological chemistry · 2013Article
- TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation.Molecular and cellular biology · 2009Article
- Computer-based screening of functional conformers of proteins.PLoS computational biology · 2008Article
- Immediate-early and delayed primary response genes are distinct in function and genomic architecture.The Journal of biological chemistry · 2007Article
- Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 (Xa5) in comparison with its homolog TFIIAgamma1.Molecular genetics and genomics : MGG · 2006Article
- Mapping and functional characterization of the TAF11 interaction with TFIIA.Molecular and cellular biology · 2005Article
- cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis.Nucleic acids research · 2005Article
- Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex.Molecular and cellular biology · 2004Article
- Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA.Genes & development · 2004Article
- A TATA binding protein mutant with increased affinity for DNA directs transcription from a reversed TATA sequence in vivo.Molecular and cellular biology · 2002Article
- The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology.Journal of bacteriology · 2001Article
- TFIIA interacts with TFIID via association with TATA-binding protein and TAF40.Molecular and cellular biology · 2001Article
- Remodeling of yeast CUP1 chromatin involves activator-dependent repositioning of nucleosomes over the entire gene and flanking sequences.Molecular and cellular biology · 2001Article
- An initiation element in the yeast CUP1 promoter is recognized by RNA polymerase II in the absence of TATA box-binding protein if the DNA is negatively supercoiled.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Activation of transcription can occur by the facilitated recruitment of TFIID to promoters by gene-specific activators. To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class of yeast TATA-binding protein (TBP) mutants that is activation and DNA binding defective. We found that co-overexpression of TOA1 and TOA2, the genes that encode yeast TFIIA, overcomes the activation defects caused by the TBP mutants. Using a genetic screen, we isolated a new class of TFIIA mutants and identified three regions on TFIIA that are likely to be involved in TBP recruitment or stabilization of the TBP-TATA complex in vivo. Amino acid replacements in only one of these regions enhance TFIIA-TBP-DNA complex formation in vitro, suggesting that the other regions are involved in regulatory interactions. To determine the relative importance of TFIIA in the regulation of different genes, we constructed yeast strains to conditionally deplete TFIIA levels prior to gene activation. While the activation of certain genes, such as INO1, was dramatically impaired by TFIIA depletion, activation of other genes, such as CUP1, was unaffected. These data suggest that TFIIA facilitates DNA binding by TBP in vivo, that TFIIA may be regulated by factors that target distinct regions of the protein, and that promoters vary significantly in the degree to which they require TFIIA for activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.