ArticleMolecular and cellular biology1999
NF-Y associates with H3-H4 tetramers and octamers by multiple mechanisms.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- PhpCNature communications · 2025Article
- Role of the Heme Activator Protein Complex in the Sexual Development of Cryptococcus neoformans.mSphere · 2022Article
- NF-Y coassociates with FOS at promoters, enhancers, repetitive elements, and inactive chromatin regions, and is stereo-positioned with growth-controlling transcription factors.Genome research · 2013Article
- The methyltransferase SMYD3 mediates the recruitment of transcriptional cofactors at the myostatin and c-Met genes and regulates skeletal muscle atrophy.Genes & development · 2013Article
- Calcium channel blockers act through nuclear factor Y to control transcription of key cardiac genes.Molecular pharmacology · 2012Article
- Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals.Molecular biology of the cell · 2012Article
- Article
- Tissue-specific expression patterns of Arabidopsis NF-Y transcription factors suggest potential for extensive combinatorial complexity.Plant physiology · 2009Article
- Nuclear factor Y is required for basal activation and chromatin accessibility of fibroblast growth factor receptor 2 promoter in osteoblast-like cells.The Journal of biological chemistry · 2009Article
- Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity.Molecular biology of the cell · 2008Article
- NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations.Nucleic acids research · 2008Article
- NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue.PloS one · 2008Article
- A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response.Nucleic acids research · 2008Article
- The histone-like NF-Y is a bifunctional transcription factor.Molecular and cellular biology · 2008Article
- Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters.Nucleic acids research · 2006Article
- Subunits of the heterotrimeric transcription factor NF-Y are imported into the nucleus by distinct pathways involving importin beta and importin 13.Molecular and cellular biology · 2005Article
- Expression of the CCAAT-binding factor NF-Y in Caenorhabditis elegans.Journal of molecular histology · 2005Article
- Np95 is a histone-binding protein endowed with ubiquitin ligase activity.Molecular and cellular biology · 2004Article
- Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- Requirement for down-regulation of the CCAAT-binding activity of the NF-Y transcription factor during skeletal muscle differentiation.Molecular biology of the cell · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
NF-Y is a CCAAT-binding trimer with two histonic subunits, NF-YB and NF-YC, resembling H2A-H2B. We previously showed that the short conserved domains of NF-Y efficiently bind to the major histocompatibility complex class II Ea Y box in DNA nucleosomized with purified chicken histones. Using wild-type NF-Y and recombinant histones, we find that NF-Y associates with H3-H4 early during nucleosome assembly, under conditions in which binding to naked DNA is not observed. In such assays, the NF-YB-NF-YC dimer forms complexes with H3-H4, for whose formation the CCAAT box is not required. We investigated whether they represent octamer-like structures, using DNase I, micrococcal nuclease, and exonuclease III, and found a highly positioned nucleosome on Ea, whose boundaries were mapped; addition of NF-YB-NF-YC does not lead to the formation of octameric structures, but changes in the digestion patterns are observed. NF-YA can bind to such preformed DNA complexes in a CCAAT-dependent way. In the absence of DNA, NF-YB-NF-YC subunits bind to H3-H4, but not to H2A-H2B, through the NF-YB histone fold. These results indicate that (i) the NF-Y histone fold dimer can efficiently associate DNA during nucleosome formation; (ii) it has an intrinsic affinity for H3-H4 but does not form octamers; and (iii) the interactions between NF-YA, NF-YB-NF-YC, and H3-H4 or nucleosomes are not mutually exclusive. Thus, NF-Y can intervene at different steps during nucleosome formation, and this scenario might be paradigmatic for other histone fold proteins involved in gene regulation.
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