ArticleMolecular and cellular biology1999
Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 127 citations in OpenAlex.
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- Article
- CHFR regulates chemoresistance in triple-negative breast cancer through destabilizing ZEB1.Cell death & disease · 2021Article
- Zeb1 promotes corneal neovascularization by regulation of vascular endothelial cell proliferation.Communications biology · 2020Article
- C-Terminal Binding Proteins Promote Neurogenesis and Oligodendrogenesis in the Subventricular Zone.Frontiers in cell and developmental biology · 2020Article
- ZEB1/NuRD complex suppresses TBC1D2b to stimulate E-cadherin internalization and promote metastasis in lung cancer.Nature communications · 2019Article
- Rationale for targeting BCL6 inGenes & development · 2019Article
- CtBP promotes metastasis of breast cancer through repressing cholesterol and activating TGF-β signaling.Oncogene · 2019Article
- miR-539-3P inhibits proliferation and invasion of gastric cancer cells by targeting CTBP1.International journal of clinical and experimental pathology · 2019Article
- CPP-E1A fusion peptides inhibit CtBP-mediated transcriptional repression.Molecular oncology · 2018Article
- α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.Cell death & disease · 2018Article
- ZEB1 confers chemotherapeutic resistance to breast cancer by activating ATM.Cell death & disease · 2018Article
- The Role of CtBP1 in Oncogenic Processes and Its Potential as a Therapeutic Target.Molecular cancer therapeutics · 2017Review
- Silencing of CtBP1 suppresses the migration in human glioma cells.Journal of molecular histology · 2016Article
- The miR-200 family and its targets regulate type II cell differentiation in human fetal lung.The Journal of biological chemistry · 2015Article
- SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer.Molecular oncology · 2015Article
- miR-205 acts as a tumour radiosensitizer by targeting ZEB1 and Ubc13.Nature communications · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
deltaEF1, a representative of the zinc finger-homeodomain protein family, is a transcriptional repressor which binds E2-box (CACCTG) and related sequences and counteracts the activators through transrepression mechanisms. It has been shown that the N-proximal region of the protein is involved in the transrepression. Here we demonstrate that deltaEF1 has a second mechanism of transrepression recruiting CtBP1 or CtBP2 as its corepressor. A two-hybrid screen of mouse cDNAs with various portions of deltaEF1 identified these proteins, which bind to deltaEF1 in a manner dependent on the PLDLSL sequence located in the short medial (MS) portion of deltaEF1. CtBP1 is the mouse orthologue of human CtBP, known as the C-terminal binding protein of adenovirus E1A, while CtBP2 is the second homologue. Fusion of mouse CtBP1 or CtBP2 to Gal4DBD (Gal4 DNA binding domain) made them Gal4 binding site-dependent transcriptional repressors in transfected 10T1/2 cells, indicating their involvement in a transcriptional repression mechanism. When the MS portion of deltaEF1 was used to Gal4DBD and used to transfect cells, a strong transrepression activity was generated, but this activity was totally dependent on the PLDLSL sequence which served as the site for interaction with endogenous CtBP proteins, indicating that CtBP1 and -2 can act as corepressors. Exogenous CtBP1/2 significantly enhanced transcriptional repression by deltaEF1, and this enhancement was lost if the PLDLSL sequence was altered, demonstrating that CtBP1 and -2 act as corepressors of deltaEF1. In the mouse, CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. In developing embryos, CtBP1 and CtBP2 are expressed broadly with different tissue preferences. Remarkably, their high expression occurs in subsets of deltaEF1-expressing tissues, e.g., cephalic and dorsal root ganglia, spinal cord, posterior-distal halves of the limb bud mesenchyme, and perichondrium of forming digits, supporting the conclusion that CtBP1 and -2 play crucial roles in the repressor action of deltaEF1 in these tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.