ArticleMolecular and cellular biology1999
Small cis-acting sequences that specify secondary structures in a chloroplast mRNA are essential for RNA stability and translation.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 110 citations in OpenAlex.
- Deep learning-based investigation of chloroplast translation regulatory sequences.Frontiers in plant science · 2025Article
- Rock, scissors, paper: How RNA structure informs function.The Plant cell · 2023Article
- Promoter activity and transcriptome analyses decipher functions of CgbHLH001 gene (Chenopodium glaucum L.) in response to abiotic stress.BMC plant biology · 2023Article
- RNA Degradation in Neurodegenerative Disease.Advances in neurobiology · 2018Review
- The RNA Structure of cis-acting Translational Elements of the Chloroplast psbC mRNA in Chlamydomonas reinhardtii.Frontiers in plant science · 2016Article
- Small RNAs reveal two target sites of the RNA-maturation factor Mbb1 in the chloroplast of Chlamydomonas.Nucleic acids research · 2014Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Messenger RNA degradation is initiated at the 5' end and follows sequence- and condition-dependent modes in chloroplasts.Nucleic acids research · 2011Article
- Article
- Downstream promoter sequence of an Indian isolate of Rice tungro bacilliform virus alters tissue-specific expression in host rice and acts differentially in heterologous system.Plant molecular biology · 2007Article
- Article
- The nucleus-encoded factor MCD4 participates in degradation of nonfunctional 3' UTR sequences generated by cleavage of pre-mRNA in Chlamydomonas chloroplasts.Molecular genetics and genomics : MGG · 2007Article
- Distinct roles for the 5' and 3' untranslated regions in the degradation and accumulation of chloroplast tufA mRNA: identification of an early intermediate in the in vivo degradation pathway.Plant molecular biology · 2007Article
- Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species.Plant molecular biology · 2006Article
- Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon.Nucleic acids research · 2006Article
- Contribution of 5'- and 3'-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes.Molecular genetics and genomics : MGG · 2005Article
- RNA-protein complexes that form in the spinach chloroplast atpI 5' untranslated region can be divided into two subcomplexes, each comprised of unique cis-elements and trans-factors.Current genetics · 2005Article
- Microarray analysis confirms the specificity of a Chlamydomonas reinhardtii chloroplast RNA stability mutant.Plant physiology · 2005Article
- Beyond complementation. Map-based cloning in Chlamydomonas reinhardtii.Plant physiology · 2005Article
- Paths toward algal genomics.Plant physiology · 2005Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Nucleus-encoded proteins interact with cis-acting elements in chloroplast transcripts to promote RNA stability and translation. We have analyzed the structure and function of three such elements within the Chlamydomonas petD 5' untranslated region; petD encodes subunit IV of the cytochrome b(6)/f complex. These elements were delineated by linker-scanning mutagenesis, and RNA secondary structures were investigated by mapping nuclease-sensitive sites in vitro and by in vivo dimethyl sulfate RNA modification. Element I spans a maximum of 8 nucleotides (nt) at the 5' end of the mRNA; it is essential for RNA stability and plays a role in translation. This element appears to form a small stem-loop that may interact with a previously described nucleus-encoded factor to block 5'-->3' exoribonucleolytic degradation. Elements II and III, located in the center and near the 3' end of the 5' untranslated region, respectively, are essential for translation, but mutations in these elements do not affect mRNA stability. Element II is a maximum of 16 nt in length, does not form an obvious secondary structure, and appears to bind proteins that protect it from dimethyl sulfate modification. Element III spans a maximum of 14 nt and appears to form a stem-loop in vivo, based on dimethyl sulfate modification and the sequences of intragenic suppressors of element III mutations. Furthermore, mutations in element II result in changes in the RNA structure near element III, consistent with a long-range interaction that may promote translation.
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