ArticleMolecular and cellular biology1999
Nuclear retention elements of U3 small nucleolar RNA.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- SnoRNAs: Exploring Their Implication in Human Diseases.International journal of molecular sciences · 2024Review
- A novel RNA motif mediates the strict nuclear localization of a long noncoding RNA.Molecular and cellular biology · 2014Article
- Intron-3 retention/splicing controls neuronal expression of apolipoprotein E in the CNS.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Involvement of nuclear import and export factors in U8 box C/D snoRNP biogenesis.Molecular and cellular biology · 2007Article
- Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans.BMC molecular biology · 2007Article
- Role of pre-rRNA base pairing and 80S complex formation in subnucleolar localization of the U3 snoRNP.Molecular and cellular biology · 2004Article
- An H/ACA guide RNA directs U2 pseudouridylation at two different sites in the branchpoint recognition region in Xenopus oocytes.RNA (New York, N.Y.) · 2002Article
- Large-scale isolation of Cajal bodies from HeLa cells.Molecular biology of the cell · 2002Article
- Two splice variants of Nopp140 in Drosophila melanogaster.Molecular biology of the cell · 2002Article
- Small nucleolar RNAs: versatile trans-acting molecules of ancient evolutionary origin.Gene expression · 2002Review
- The snoRNA domain of vertebrate telomerase RNA functions to localize the RNA within the nucleus.RNA (New York, N.Y.) · 2001Article
- An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association.RNA (New York, N.Y.) · 2001Article
- Internal modification of U2 small nuclear (sn)RNA occurs in nucleoli of Xenopus oocytes.The Journal of cell biology · 2001Article
- Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.Nucleic acids research · 2001Review
- snoRNA nuclear import and potential for cotranscriptional function in pre-rRNA processing.RNA (New York, N.Y.) · 2001Article
- The box C/D motif directs snoRNA 5'-cap hypermethylation.Nucleic acids research · 2000Article
- The matrix protein of vesicular stomatitis virus inhibits nucleocytoplasmic transport when it is in the nucleus and associated with nuclear pore complexes.Molecular and cellular biology · 2000Article
- Interaction of the U3-55k protein with U3 snoRNA is mediated by the box B/C motif of U3 and the WD repeats of U3-55k.Nucleic acids research · 2000Article
- Transient nucleolar localization Of U6 small nuclear RNA in Xenopus Laevis oocytes.Molecular biology of the cell · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The processing and methylation of precursor rRNA is mediated by the box C/D small nucleolar RNAs (snoRNAs). These snoRNAs differ from most cellular RNAs in that they are not exported to the cytoplasm. Instead, these RNAs are actively retained in the nucleus where they assemble with proteins into mature small nucleolar ribonucleoprotein particles and are targeted to their intranuclear site of action, the nucleolus. In this study, we have identified the cis-acting sequences responsible for the nuclear retention of U3 box C/D snoRNA by analyzing the nucleocytoplasmic distributions of an extensive panel of U3 RNA variants after injection of the RNAs into Xenopus oocyte nuclei. Our data indicate the importance of two conserved sequence motifs in retaining U3 RNA in the nucleus. The first motif is comprised of the conserved box C' and box D sequences that characterize the box C/D family. The second motif contains conserved box sequences B and C. Either motif is sufficient for nuclear retention, but disruption of both motifs leads to mislocalization of the RNAs to the cytoplasm. Variant RNAs that are not retained also lack 5' cap hypermethylation and fail to associate with fibrillarin. Furthermore, our results indicate that nuclear retention of U3 RNA does not simply reflect its nucleolar localization. A fragment of U3 containing the box B/C motif is not localized to nucleoli but retained in coiled bodies. Thus, nuclear retention and nucleolar localization are distinct processes with differing sequence requirements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.