ArticleMolecular and cellular biology1999
Coexamination of site-specific transcription factor binding and promoter activity in living cells.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
76 citing papers in PubMed, 165 citations in OpenAlex.
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- Argonaute 4 as an Effector Protein in RNA-Directed DNA Methylation in Human Cells.Frontiers in genetics · 2019Article
- Standardizing chromatin research: a simple and universal method for ChIP-seq.Nucleic acids research · 2016Article
- Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation.Cancer science · 2015Article
- c-MYC Generates Repair Errors via Increased Transcription of Alternative-NHEJ Factors, LIG3 and PARP1, in Tyrosine Kinase-Activated Leukemias.Molecular cancer research : MCR · 2015Article
- MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1.Molecular and cellular biology · 2014Article
- IFI16 restricts HSV-1 replication by accumulating on the hsv-1 genome, repressing HSV-1 gene expression, and directly or indirectly modulating histone modifications.PLoS pathogens · 2014Article
- Kaposi's sarcoma-associated herpesvirus LANA recruits the DNA polymerase clamp loader to mediate efficient replication and virus persistence.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- O-GlcNAcylation and p50/p105 binding of c-Rel are dynamically regulated by LPS and glucosamine in BV2 microglia cells.British journal of pharmacology · 2013Article
- Transcription-coupled eviction of histones H2A/H2B governs V(D)J recombination.The EMBO journal · 2013Article
- Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells.Toxicology and applied pharmacology · 2013Article
- Downregulation of histone methyltransferase genes SUV39H1 and SUV39H2 increases telomere length in embryonic stem-like cells and embryonic fibroblasts in pigs.The Journal of reproduction and development · 2013Article
- The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration.Molecular and cellular biology · 2012Article
- Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2.PloS one · 2012Article
- Transcriptional regulation of hTREX84 in human cancer cells.PloS one · 2012Article
- FoxO3A promotes metabolic adaptation to hypoxia by antagonizing Myc function.The EMBO journal · 2011Article
- Gadd45β is an inducible coactivator of transcription that facilitates rapid liver growth in mice.The Journal of clinical investigation · 2011Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previously, we have used a chromatin cross-linking and immunoprecipitation protocol for the analysis of Myc and USF binding to the cad promoter. The adaptation of this technique for the study of mammalian transcription factors was a big step forward in the analysis of transcription factor family member specificity, allowing for the first time a definitive knowledge of which factor binds to a promoter region under normal physiological conditions. However, due to limitations of the assay, our previous studies could not definitively prove that both Myc and USF bound to the exact same site on the cad promoter, nor could we directly correlate loss of in vivo binding of a particular factor with loss of transcriptional activity. Therefore, we have further modified the chromatin immunoprecipitation protocol to alleviate these problems. We have now shown that it is possible to coexamine growth-regulated transcriptional activity and promoter occupancy by using stably integrated promoter constructs. We show that both Myc and USF bind to the exact same E box on the cad promoter, suggesting that competition between these two factors for a single site occurs in living cells. We also find that cad promoter constructs that retain USF binding but lose Myc binding in vivo no longer display an increase in transcriptional activity in mid- to late G(1) phase of the cell cycle. Finally, we propose that cell cycle-regulated transcriptional activation of the cad promoter may be a stochastic, rather than a predetermined, process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.