ArticleMolecular and cellular biology1999
Direct binding and In vivo regulation of the fission yeast p21-activated kinase shk1 by the SH3 domain protein scd2.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- MoScd2 is involved in appressorium formation and pathogenicity via the Pmk1 MAPK pathway in Magnaporthe oryzae.Crop health · 2023Article
- High-throughput RNA sequencing reveals differences between the transcriptomes of the five spore forms of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen.Stress biology · 2023Article
- Cdc42 GTPase-activating proteins (GAPs) regulate generational inheritance of cell polarity and cell shape in fission yeast.Molecular biology of the cell · 2021Article
- Article
- Review
- A time-resolved interaction analysis of Bem1 reconstructs the flow of Cdc42 during polar growth.Life science alliance · 2020Article
- Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase.PLoS biology · 2020Article
- Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.Journal of cell science · 2018Article
- Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth.Science (New York, N.Y.) · 2012Article
- PAK family kinases: Physiological roles and regulation.Cellular logistics · 2012Article
- Spatial control of Cdc42 activation determines cell width in fission yeast.Molecular biology of the cell · 2011Article
- Role of Hsl7 in morphology and pathogenicity and its interaction with other signaling components in the plant pathogen Ustilago maydis.Eukaryotic cell · 2011Article
- Two CDC42 paralogues modulate Cryptococcus neoformans thermotolerance and morphogenesis under host physiological conditions.Molecular microbiology · 2010Article
- Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.Molecular biology of the cell · 2009Article
- PAK thread from amoeba to mammals.Journal of cellular biochemistry · 2009Review
- Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis.The EMBO journal · 2007Article
- Article
- Interactions between Cdc42 and the scaffold protein Scd2: requirement of SH3 domains for GTPase binding.The Biochemical journal · 2005Article
- Interaction with the SH3 domain protein Bem1 regulates signaling by the Saccharomyces cerevisiae p21-activated kinase Ste20.Molecular and cellular biology · 2005Article
- Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis.Genetics · 2003Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The Ste20/p21-activated kinase homolog Shk1 is essential for viability and required for normal morphology, mating, and cell cycle control in the fission yeast Schizosaccharomyces pombe. Shk1 is regulated by the p21 G protein Cdc42, which has been shown to form a complex with the SH3 domain protein Scd2 (also called Ral3). In this study, we investigated whether Scd2 plays a role in regulating Shk1 function. We found that recombinant Scd2 and Shk1 interact directly in vitro and that they interact in vivo, as determined by the two-hybrid assay and genetic analyses in fission yeast. The second of two N-terminal SH3 domains of Scd2 is both necessary and sufficient for interaction with Shk1. While full-length Scd2 interacted with only the R1 N-terminal regulatory subdomain of Shk1, a C-terminal deletion mutant of Scd2 interacted with both the R1 and R3 subdomains of Shk1, suggesting that the non-SH3 C-terminal domain of Scd2 may be involved in defining specificity in SH3 binding domain recognition. Overexpression of Scd2 stimulated the autophosphorylation activity of wild-type Shk1 in fission yeast but, consistent with results of genetic analyses, did not stimulate the activity of a Shk1 protein lacking the R1 subdomain. Results of additional two-hybrid experiments suggest that Scd2 may stimulate Shk1 catalytic function, at least in part, by positively modulating protein-protein interaction between Cdc42 and Shk1. We propose that Scd2 functions as an organizing center, or scaffold, for the Cdc42 complex in fission yeast and that it acts in concert with Cdc42 to positively regulate Shk1 function.
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