ArticleMolecular and cellular biology1999
Bni1p regulates microtubule-dependent nuclear migration through the actin cytoskeleton in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- LIS1 and DCX: Implications for Brain Development and Human Disease in Relation to Microtubules.Scientifica · 2013Review
- Yeast formin Bni1p has multiple localization regions that function in polarized growth and spindle orientation.Molecular biology of the cell · 2012Article
- Formins and microtubules.Biochimica et biophysica acta · 2010Review
- The temperature-sensitive role of Cryptococcus neoformans ROM2 in cell morphogenesis.PloS one · 2007Article
- Lissencephaly 1 linking to multiple diseases: mental retardation, neurodegeneration, schizophrenia, male sterility, and more.Neuromolecular medicine · 2006Review
- Stable and dynamic axes of polarity use distinct formin isoforms in budding yeast.Molecular biology of the cell · 2004Article
- Lrg1p Is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast.Genetics · 2004Article
- Overexpression of an Arabidopsis formin stimulates supernumerary actin cable formation from pollen tube cell membrane.The Plant cell · 2004Article
- Cytokinesis in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2002Review
- LIS1, CLIP-170's key to the dynein/dynactin pathway.Molecular and cellular biology · 2002Article
- LIS1-no more no less.Molecular psychiatry · 2002Review
- A protein interaction map for cell polarity development.The Journal of cell biology · 2001Article
- Dynamic localization and function of Bni1p at the sites of directed growth in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast.The Journal of cell biology · 2001Article
- Different domains of the essential GTPase Cdc42p required for growth and development of Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.The Journal of cell biology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RHO1 gene encodes a yeast homolog of the mammalian RhoA protein. Rho1p is localized to the growth sites and is required for bud formation. We have recently shown that Bni1p is one of the potential downstream target molecules of Rho1p. The BNI1 gene is implicated in cytokinesis and the establishment of cell polarity in Saccharomyces cerevisiae but is not essential for cell viability. In this study, we screened for mutations that were synthetically lethal in combination with a bni1 mutation and isolated two genes. They were the previously identified PAC1 and NIP100 genes, both of which are implicated in nuclear migration in S. cerevisiae. Pac1p is a homolog of human LIS1, which is required for brain development, whereas Nip100p is a homolog of rat p150(Glued), a component of the dynein-activated dynactin complex. Disruption of BNI1 in either the pac1 or nip100 mutant resulted in an enhanced defect in nuclear migration, leading to the formation of binucleate mother cells. The arp1 bni1 mutant showed a synthetic lethal phenotype while the cin8 bni1 mutant did not, suggesting that Bni1p functions in a kinesin pathway but not in the dynein pathway. Cells of the pac1 bni1 and nip100 bni1 mutants exhibited a random distribution of cortical actin patches. Cells of the pac1 act1-4 mutant showed temperature-sensitive growth and a nuclear migration defect. These results indicate that Bni1p regulates microtubule-dependent nuclear migration through the actin cytoskeleton. Bni1p lacking the Rho-binding region did not suppress the pac1 bni1 growth defect, suggesting a requirement for the Rho1p-Bni1p interaction in microtubule function.
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