ArticleMolecular and cellular biology1999
Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 201 citations in OpenAlex.
- Minichromosome maintenance 4 is associated with poor survival and stemness of patients with pancreatic cancer.Medical molecular morphology · 2026Article
- Review
- Viperin catalyzes methionine oxidation to promote protein expression and function of helicases.Science advances · 2019Article
- Identification of Key Genes and Pathways in Cervical Cancer by Bioinformatics Analysis.International journal of medical sciences · 2019Article
- O-GlcNAc transferase associates with the MCM2-7 complex and its silencing destabilizes MCM-MCM interactions.Cellular and molecular life sciences : CMLS · 2018Article
- Kaposi's Sarcoma-Associated Herpesvirus Deregulates Host Cellular Replication during Lytic Reactivation by Disrupting the MCM Complex through ORF59.Journal of virology · 2018Article
- The Human Replicative Helicase, the CMG Complex, as a Target for Anti-cancer Therapy.Frontiers in molecular biosciences · 2018Review
- Potent DNA strand annealing activity associated with mouse Mcm2∼7 heterohexameric complex.Nucleic acids research · 2017Article
- iTRAQ-based Proteomic Analysis of Porcine Kidney Epithelial PK15 cells Infected with Pseudorabies virus.Scientific reports · 2017Article
- RNAi-mediated knockdown of MCM7 gene on CML cells and its therapeutic potential for leukemia.Medical oncology (Northwood, London, England) · 2017Article
- Arabidopsis thaliana MCM3 single subunit of MCM2-7 complex functions as 3' to 5' DNA helicase.Protoplasma · 2016Article
- The DNA replication licensing factor miniature chromosome maintenance 7 is essential for RNA splicing of epidermal growth factor receptor, c-Met, and platelet-derived growth factor receptor.The Journal of biological chemistry · 2015Article
- Geminin inhibits a late step in the formation of human pre-replicative complexes.The Journal of biological chemistry · 2014Article
- Identification of genes with altered expression in male and female Schlager hypertensive mice.BMC medical genetics · 2014Article
- Whole adult organism transcriptional profiling of acute metal exposures in male zebrafish.BMC pharmacology & toxicology · 2014Article
- MCM Paradox: Abundance of Eukaryotic Replicative Helicases and Genomic Integrity.Molecular biology international · 2014Review
- Interaction of MCM7 and RACK1 for activation of MCM7 and cell growth.The American journal of pathology · 2013Article
- Article
- Promoter of a salinity and cold stress-induced MCM6 DNA helicase from pea.Plant signaling & behavior · 2011Article
- Molecular mechanism of activation of human Cdc7 kinase: bipartite interaction with Dbf4/activator of S phase kinase (ASK) activation subunit stimulates ATP binding and substrate recognition.The Journal of biological chemistry · 2011Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mcm proteins play an essential role in eukaryotic DNA replication, but their biochemical functions are poorly understood. Recently, we reported that a DNA helicase activity is associated with an Mcm4-Mcm6-Mcm7 (Mcm4,6,7) complex, suggesting that this complex is involved in the initiation of DNA replication as a DNA-unwinding enzyme. In this study, we have expressed and isolated the mouse Mcm2, 4,6,7 proteins from insect cells and characterized various mutant Mcm4,6,7 complexes in which the conserved ATPase motifs of the Mcm4 and Mcm6 proteins were mutated. The activities associated with such preparations demonstrated that the DNA helicase activity is intrinsically associated with the Mcm4,6,7 complex. Biochemical analyses of these mutant Mcm4,6,7 complexes indicated that the ATP binding activity of the Mcm6 protein in the complex is critical for DNA helicase activity and that the Mcm4 protein may play a role in the single-stranded DNA binding activity of the complex. The results also indicated that the two activities of DNA helicase and single-stranded DNA binding can be separated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.