ArticleMolecular and cellular biology1999
Translation of maternal TATA-binding protein mRNA potentiates basal but not activated transcription in Xenopus embryos at the midblastula transition.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 85 citations in OpenAlex.
- Article
- IMPACTS OF DNA METHYLATION ON H2A.Z DEPOSITION AND NUCLEOSOME STABILITY.bioRxiv : the preprint server for biology · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Review
- Article
- The miR-430 locus with extreme promoter density forms a transcription body during the minor wave of zygotic genome activation.Developmental cell · 2023Article
- Article
- The physiological and pathological mechanisms of early embryonic development.Fundamental research · 2022Review
- Nuclear export restricts Gdown1 to a mitotic function.Nucleic acids research · 2022Article
- What defines the maternal transcriptome?Biochemical Society transactions · 2021Review
- The nuclear to cytoplasmic ratio directly regulates zygotic transcription inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Control of zygotic genome activation in Xenopus.Current topics in developmental biology · 2021Review
- Mechanisms regulating zygotic genome activation.Nature reviews. Genetics · 2019Review
- Regulatory principles governing the maternal-to-zygotic transition: insights from Drosophila melanogaster.Open biology · 2018Review
- Post-translational regulation of the maternal-to-zygotic transition.Cellular and molecular life sciences : CMLS · 2018Review
- Flying the RNA Nest: Drosophila Reveals Novel Insights into the Transcriptome Dynamics of Early Development.Journal of developmental biology · 2018Review
- Zygotic Genome Activation in Vertebrates.Developmental cell · 2017Review
- Article
- Activation of a T-box-Otx2-Gsc gene network independent of TBP and TBP-related factors.Development (Cambridge, England) · 2016Article
- Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Early embryonic development in Xenopus laevis is characterized by transcriptional repression which is relieved at the midblastula stage (MBT). Here we show that the relative abundance of TATA-binding protein (TBP) increases robustly at the MBT and that the mechanism underlying this increase is translation of maternally stored TBP RNA. We show that TBP is rate-limiting in egg extract under conditions that titrate nucleosome assembly. Precocious translation of TBP mRNA in Xenopus embryos facilitates transcription before the MBT, without requiring TBP to be prebound to the promoter before injection. This effect is transient in the absence of chromatin titration and is sustained when chromatin is titrated. These data show that translational regulation of TBP RNA contributes to limitations on the transcriptional capacity before the MBT. Second, we examined the ability of trans-acting factors to contribute to promoter activity before the MBT. Deletion of cis-acting elements does not affect histone H2B transcription in egg extract, a finding indicative of limited trans-activation. Moreover, in the context of the intact promoter, neither the transcriptional activator Oct-1, nor TBP, nor TFIID enable transcriptional activation in vitro. HeLa cell extract, however, reconstitutes activated transcription in mixed extracts. These data suggest a deficiency in egg extract cofactors required for activated transcription. We show that the capacity for activated H2B transcription is gradually acquired at the early gastrula transition. This transition occurs well after the blastula stage when the basal transcription machinery can first be complemented with TBP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.