ArticleThe Biochemical journal1999
Identification of MUC5B, MUC5AC and small amounts of MUC2 mucins in cystic fibrosis airway secretions.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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Who cites it
45 citing papers in PubMed, 109 citations in OpenAlex.
- How Does Biological Sex Impact Mucosal Bacterial Infection? Mucosal Defenses and Bacterial Detection.Immunological reviews · 2026Review
- The FOXA transcription factors in allergic airway disease and asthma.Frontiers in allergy · 2026Review
- Host- and microbial-mediated mucin degradation differentially shape Pseudomonas aeruginosa physiology and gene expression.PLoS pathogens · 2025Article
- Transcriptional profiling of mucus production in rhesus macaque endocervical cells under hormonal regulation†.Biology of reproduction · 2024Article
- Advances in the Evaluation of Gastrointestinal Absorption Considering the Mucus Layer.Pharmaceutics · 2023Review
- Mucociliary Respiratory Epithelium Integrity in Molecular Defense and Susceptibility to Pulmonary Viral Infections.Biology · 2021Review
- Mechanism of azithromycin in airway diseases.The Journal of international medical research · 2020Review
- Article
- The C-terminal dimerization domain of the respiratory mucin MUC5B functions in mucin stability and intracellular packaging before secretion.The Journal of biological chemistry · 2019Article
- Infection Is Not Required for Mucoinflammatory Lung Disease in CFTR-Knockout Ferrets.American journal of respiratory and critical care medicine · 2018Article
- Physicochemical properties of mucus and their impact on transmucosal drug delivery.International journal of pharmaceutics · 2017Review
- Aspergillosis and the role of mucins in cystic fibrosis.Pediatric pulmonology · 2017Review
- miR-263a Regulates ENaC to Maintain Osmotic and Intestinal Stem Cell Homeostasis in Drosophila.Developmental cell · 2017Article
- Complement effectors, C5a and C3a, in cystic fibrosis lung fluid correlate with disease severity.PloS one · 2017Article
- Inhibition of Myeloperoxidase Activity in Cystic Fibrosis Sputum by Peptide Inhibitor of Complement C1 (PIC1).PloS one · 2017Article
- Article
- Idiopathic Pulmonary Fibrosis: A Genetic Disease That Involves Mucociliary Dysfunction of the Peripheral Airways.Physiological reviews · 2016Review
- Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization.Annals of the American Thoracic Society · 2016Article
- Complement Effectors of Inflammation in Cystic Fibrosis Lung Fluid Correlate with Clinical Measures of Disease.PloS one · 2015Article
- More than one disease process in chronic sinusitis based on mucin fragmentation patterns and amino Acid analysis.International journal of otolaryngology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The 'insoluble' residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between the different individuals was low. Antibodies recognizing MUC5AC and MUC5B identified these mucins in each of the fractions. MUC2, however, was present only as a component of the insoluble residue from the gel which accounted for less than 4% by mass of the total mucins. MUC5B and MUC5AC from the gel phase were large oligomeric species composed of disulphide-bond linked subunits and MUC5B was present as two populations with different charge densities which are likely to correspond to MUC5B 'glycoforms'. The sol phase contained, in addition to MUC5AC and MUC5B, mainly smaller mucins which did not react with the antisera and which were probably degraded. MUC5AC appeared to be enriched in the sol, suggesting that this mucin may be more susceptible to proteolytic degradation than MUC5B. The mucins present in sputum remained broadly similar during acute exacerbation and following antibiotic treatment, although the relative amount of an acidic MUC5B glycoform was decreased during infection.
Indexed as
Identifiers
10567212PMC1220647W2136143572What OpenQuestion holds
Registered trials
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