SynthesisJournal of clinical microbiology1999
Performance criteria of DNA fingerprinting methods for typing of Helicobacter pylori isolates: experimental results and meta-analysis.
Synthesis in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Recurrence Rate and Influencing Factors of Helicobacter Pylori Infection After Successful Eradication in Southern Coastal China.International journal of general medicine · 2024Article
- Comparative Assessment of Different PCR-Based Typing Methods ofInfection and drug resistance · 2021Article
- Heterogeneity ofInfection and drug resistance · 2021Article
- Where to Biopsy to DetectJournal of clinical medicine · 2020Article
- [Research progress on genotyping ofZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2018Review
- Comparison of virulence gene profiles and genomic fingerprints ofTropical medicine and health · 2018Article
- CRISPR-like sequences inGut pathogens · 2017Article
- Application of the Random Amplified Polymorphic DNA (RAPD) Fingerprinting to Analyze Genetic Variation in Community Associated-Methicillin Resistant Staphylococcus Aureus (CA-MRSA) Isolates in Iran.Global journal of health science · 2016Article
- Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR.Journal of clinical and diagnostic research : JCDR · 2015Article
- Clinical Application of the DiversiLab Microbial Typing System Using Repetitive Sequence-Based PCR for Characterization of Helicobacter pylori in Japan.Journal of clinical laboratory analysis · 2015Article
- Article
- Molecular epidemiologic analysis and antimicrobial resistance of Helicobacter cinaedi isolated from seven hospitals in Japan.Journal of clinical microbiology · 2012Article
- Multiple infection and microdiversity among Helicobacter pylori isolates in a single host in India.PloS one · 2012Article
- Prevalence of Helicobacter pullorum in conventional, organic, and free-range broilers and typing of isolates.Applied and environmental microbiology · 2011Article
- Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA.Gastroenterology research and practice · 2011Article
- PCR melting profile (PCR MP)--a new tool for differentiation of Candida albicans strains.BMC infectious diseases · 2009Article
- Comparative genomics of Helicobacter pylori.World journal of gastroenterology · 2009Review
- Restriction fragment length polymorphism of urease C and urease B genes of Helicobacter pylori strains isolated from Brazilian patients with peptic ulcer and chronic gastritis.Digestive diseases and sciences · 2009Article
- Rapid determination of Escherichia coli O157:H7 lineage types and molecular subtypes by using comparative genomic fingerprinting.Applied and environmental microbiology · 2008Article
- Helicobacter pylori detection and antimicrobial susceptibility testing.Clinical microbiology reviews · 2007Review
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3 authors.
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Abstract
Typing systems are used to discriminate between isolates of Helicobacter pylori for epidemiological and clinical purposes. Discriminatory power and typeability are important performance criteria of typing systems. Discriminatory power refers to the ability to differentiate among unrelated isolates; it is quantitatively expressed by the discriminatory index (DI). Typeability refers to the ability of the method to provide an unambiguous result for each isolate analyzed; it is quantitatively expressed by the percentage of typeable isolates. We evaluated the discriminatory power and the typeability of the most currently used DNA fingerprinting methods for the typing of H. pylori isolates: ribotyping, PCR-based restriction fragment length polymorphism (PCR-RFLP) analysis, and random amplified polymorphism DNA (RAPD) analysis. Forty epidemiologically unrelated clinical isolates were selected to constitute a test population adapted to the evaluation of these performance criteria. A meta-analysis of typeability and discriminatory power was conducted retrospectively with raw data from published studies in which ribotyping, PCR-RFLP, RAPD, repetitive extragenic palindromic DNA sequence-based PCR (REP-PCR), or pulsed-field gel electrophoresis (PFGE) was used. Experimental results and the meta-analysis demonstrated the optimal typeability (100%) and the excellent discriminatory powers of PCR-based typing methods: RAPD analysis, DIs, 0.99 to 1; REP-PCR, DI, 0.99; and PCR-RFLP analysis, DIs, 0.70 to 0.97). Chromosome restriction-based typing methods (ribotyping and PFGE) are limited by a low typeability (12.5 to 75%) that strongly decreases their discriminatory powers: ribotyping, DI, 0.92; PFGE, DIs, 0.24 to 0.88. We do not recommend the use of ribotyping and PFGE for the typing of H. pylori isolates. We recommend the use of PCR-based methods.
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