ArticleMolecular biology of the cell1999
Di-leucine signals mediate targeting of tyrosinase and synaptotagmin to synaptic-like microvesicles within PC12 cells.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- The road to lysosome-related organelles: Insights from Hermansky-Pudlak syndrome and other rare diseases.Traffic (Copenhagen, Denmark) · 2019Review
- Molecular Signatures Underlying Synaptic Vesicle Cargo Retrieval.Frontiers in cellular neuroscience · 2017Review
- Expression of OA1 limits the fusion of a subset of MVBs with lysosomes - a mechanism potentially involved in the initial biogenesis of melanosomes.Journal of cell science · 2013Article
- Sorting of the vesicular GABA transporter to functional vesicle pools by an atypical dileucine-like motif.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- A tandem Di-hydrophobic motif mediates clathrin-dependent endocytosis via direct binding to the AP-2 ασ2 subunits.The Journal of biological chemistry · 2012Article
- RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.The Journal of cell biology · 2010Article
- Palmitoylation-dependent association with CD63 targets the Ca2+ sensor synaptotagmin VII to lysosomes.The Journal of cell biology · 2010Article
- Hermansky-Pudlak syndrome protein complexes associate with phosphatidylinositol 4-kinase type II alpha in neuronal and non-neuronal cells.The Journal of biological chemistry · 2009Article
- Synaptic vesicle protein trafficking at the glutamate synapse.Neuroscience · 2009Review
- Trafficking of vesicular neurotransmitter transporters.Traffic (Copenhagen, Denmark) · 2008Review
- Phosphatidylinositol-4-kinase type II alpha contains an AP-3-sorting motif and a kinase domain that are both required for endosome traffic.Molecular biology of the cell · 2008Article
- Melanosomes--dark organelles enlighten endosomal membrane transport.Nature reviews. Molecular cell biology · 2007Review
- Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- BLOC-1 complex deficiency alters the targeting of adaptor protein complex-3 cargoes.Molecular biology of the cell · 2006Article
- Functions of adaptor protein (AP)-3 and AP-1 in tyrosinase sorting from endosomes to melanosomes.Molecular biology of the cell · 2005Article
- Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.Molecular biology of the cell · 2005Article
- Plasma membrane localization signals in the light chain of botulinum neurotoxin.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Melanosomes and MHC class II antigen-processing compartments: a tinted view of intracellular trafficking and immunity.Immunologic research · 2003Review
- Signals involved in targeting membrane proteins to synaptic vesicles.Cellular and molecular neurobiology · 2002Review
- Rab4 regulates formation of synaptic-like microvesicles from early endosomes in PC12 cells.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One pathway in forming synaptic-like microvesicles (SLMV) involves direct budding from the plasma membrane, requires adaptor protein 2 (AP2) and is brefeldin A (BFA) resistant. A second route leads from the plasma membrane to an endosomal intermediate from which SLMV bud in a BFA-sensitive, AP3-dependent manner. Because AP3 has been shown to bind to a di-leucine targeting signal in vitro, we have investigated whether this major class of targeting signals is capable of directing protein traffic to SLMV in vivo. We have found that a di-leucine signal within the cytoplasmic tail of human tyrosinase is responsible for the majority of the targeting of HRP-tyrosinase chimeras to SLMV in PC12 cells. Furthermore, we have discovered that a Met-Leu di-hydrophobic motif within the extreme C terminus of synaptotagmin I supports 20% of the SLMV targeting of a CD4-synaptotagmin chimera. All of the traffic to the SLMV mediated by either di-Leu or Met-Leu is BFA sensitive, strongly suggesting a role for AP3 and possibly for an endosomal intermediate in this process. The differential reduction in SLMV targeting for HRP-tyrosinase and CD4-synaptotagmin chimeras by di-alanine substitutions or BFA treatment implies that different proteins use the two routes to the SLMV to differing extents.
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