ArticleMolecular biology of the cell1999
Characterization of an in vitro model of elastic fiber assembly.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 68 citations in OpenAlex.
- Calcification of the elastic component: the impact on the cardiovascular system.Frontiers in cardiovascular medicine · 2025Review
- Sodium Nitroprusside Stimulation of Elastic Matrix Regeneration by Aneurysmal Smooth Muscle Cells.Tissue engineering. Part A · 2023Article
- Codependence of Bone Morphogenetic Protein Receptor 2 and Transforming Growth Factor-β in Elastic Fiber Assembly and Its Perturbation in Pulmonary Arterial Hypertension.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Vascular wall extracellular matrix proteins and vascular diseases.Biochimica et biophysica acta · 2014Review
- Advances in biomimetic regeneration of elastic matrix structures.Drug delivery and translational research · 2012Article
- Extracellular matrix molecules facilitating vascular biointegration.Journal of functional biomaterials · 2012Article
- Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts.The Biochemical journal · 2009Article
- Current advances in research and clinical applications of PLGA-based nanotechnology.Expert review of molecular diagnostics · 2009Review
- Fibrillins, fibulins, and matrix-associated glycoprotein modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide.Biochemistry · 2008Article
- Development of small-diameter vascular grafts.World journal of surgery · 2007Review
- Domains 16 and 17 of tropoelastin in elastic fibre formation.The Biochemical journal · 2007Article
- Molecular analysis of fibulin-5 function during de novo synthesis of elastic fibers.Molecular and cellular biology · 2007Article
- Altered vascular remodeling in fibulin-5-deficient mice reveals a role of fibulin-5 in smooth muscle cell proliferation and migration.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Expression of elastic components in healthy and varicose veins.World journal of surgery · 2003Article
- Fibrillin-rich microfibrils: elastic biopolymers of the extracellular matrix.Journal of muscle research and cell motility · 2002Review
- An open reading frame element mediates posttranscriptional regulation of tropoelastin and responsiveness to transforming growth factor beta1.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Elastic fibers consist of two morphologically distinct components: elastin and 10-nm fibrillin-containing microfibrils. During development, the microfibrils form bundles that appear to act as a scaffold for the deposition, orientation, and assembly of tropoelastin monomers into an insoluble elastic fiber. Although microfibrils can assemble independent of elastin, tropoelastin monomers do not assemble without the presence of microfibrils. In the present study, immortalized ciliary body pigmented epithelial (PE) cells were investigated for their potential to serve as a cell culture model for elastic fiber assembly. Northern analysis showed that the PE cells express microfibril proteins but do not express tropoelastin. Immunofluorescence staining and electron microscopy confirmed that the microfibril proteins produced by the PE cells assemble into intact microfibrils. When the PE cells were transfected with a mammalian expression vector containing a bovine tropoelastin cDNA, the cells were found to express and secrete tropoelastin. Immunofluorescence and electron microscopic examination of the transfected PE cells showed the presence of elastic fibers in the matrix. Biochemical analysis of this matrix showed the presence of cross-links that are unique to mature insoluble elastin. Together, these results indicate that the PE cells provide a unique, stable in vitro system in which to study elastic fiber assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.