ArticleThe Journal of physiology1999
Basolateral proteinase-activated receptor (PAR-2) induces chloride secretion in M-1 mouse renal cortical collecting duct cells.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Role of Mast Cells and Their Mediators in Chronic Kidney Diseases.International journal of molecular sciences · 2025Review
- Transmembrane Serine Protease 2 and Proteolytic Activation of the Epithelial Sodium Channel in Mouse Kidney.Journal of the American Society of Nephrology : JASN · 2025Article
- Protease-activated receptors in health and disease.Physiological reviews · 2023Review
- Prostaglandin E2 stimulates the epithelial sodium channel (ENaC) in cultured mouse cortical collecting duct cells in an autocrine manner.The Journal of general physiology · 2020Article
- Targeting zonulin and intestinal epithelial barrier function to prevent onset of arthritis.Nature communications · 2020Article
- Role of protease-activated receptor 2 in regulation of renin synthesis and secretion in mice.Naunyn-Schmiedeberg's archives of pharmacology · 2019Article
- Biomarkers and the role of mast cells as facilitators of inflammation and fibrosis in chronic kidney disease.Translational andrology and urology · 2019Review
- Inhibitors of the proteasome stimulate the epithelial sodium channel (ENaC) through SGK1 and mimic the effect of aldosterone.Pflugers Archiv : European journal of physiology · 2018Article
- Protease-activated receptors in kidney disease progression.American journal of physiology. Renal physiology · 2016Review
- Proteolytic activation of the human epithelial sodium channel by trypsin IV and trypsin I involves distinct cleavage sites.The Journal of biological chemistry · 2014Article
- Proteolytic activation of the epithelial sodium channel (ENaC) by the cysteine protease cathepsin-S.Pflugers Archiv : European journal of physiology · 2012Article
- Ion channels in inflammation.Pflugers Archiv : European journal of physiology · 2011Review
- Matriptase activation, an early cellular response to acidosis.The Journal of biological chemistry · 2010Article
- Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition.American journal of physiology. Cell physiology · 2009Article
- A voltage-dependent Ca2+ influx pathway regulates the Ca2+-dependent Cl(-) conductance of renal IMCD-3 cells.The Journal of membrane biology · 2009Article
- Promoter methylation of protease-activated receptor (PAR2) is associated with severe clinical phenotypes of ulcerative colitis (UC).Clinical and experimental medicine · 2009Article
- Plasmin in nephrotic urine activates the epithelial sodium channel.Journal of the American Society of Nephrology : JASN · 2009Article
- Proteinase-activated receptor 2 stimulates Na,K-ATPase and sodium reabsorption in native kidney epithelium.The Journal of biological chemistry · 2008Article
- Aldosterone responsiveness of the epithelial sodium channel (ENaC) in colon is increased in a mouse model for Liddle's syndrome.The Journal of physiology · 2008Article
- Protease-activated receptor-2 (PAR-2) is a weak enhancer of mucin secretion by human bronchial epithelial cells in vitro.The international journal of biochemistry & cell biology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
1. Using RT-PCR, Northern blot analysis, and immunocytochemistry, we confirmed renal expression of proteinase-activated receptor (PAR-2) and demonstrated its presence in native renal epithelial and in cultured M-1 mouse cortical collecting duct (CCD) cells. 2. We investigated the effects of a PAR-2 activating peptide (AP), corresponding to the tethered ligand that is exposed upon trypsin cleavage, and of trypsin on M-1 cells using patch-clamp, intracellular calcium (fura-2) and transepithelial short-circuit current (ISC) measurements. 3. In single M-1 cells, addition of AP elicited a concentration-dependent transient increase in the whole-cell conductance. Removal of extracellular Na+ had no effect while removal of Cl- prevented the stimulation of outward currents. The intracellular calcium concentration increased significantly upon application of AP while a Ca2+-free pipette solution completely abolished the electrical response to AP. 4. In confluent monolayers of M-1 cells, apical application of AP had no effect on ISC whereas subsequent basolateral application elicited a transient increase in ISC. This increase was not due to a stimulation of electrogenic Na+ absorption since the response was preserved in the presence of amiloride. 5. The ISC response to AP was reduced in the presence of the Cl- channel blocker diphenylamine-2-carboxylic acid on the apical side and abolished in the absence of extracellular Cl-. 6. Trypsin elicited similar responses to those to AP while application of a peptide (RP) with the reverse amino acid sequence of AP had no effect on whole-cell currents or ISC. 7. In conclusion, our data suggest that AP or trypsin stimulates Cl- secretion by Ca2+-activated Cl- channels in M-1 CCD cells by activating basolateral PAR-2.
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