ArticlePlant molecular biology1999
A new class of proteins capable of binding transition metals.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Two folds, many faces: The Magnaporthe oryzae MAX effector AVR-Pia targets novel rice HMA domain-containing proteins.PLoS pathogens · 2026Article
- A key heavy metal-binding protein orchestrates plant resistance against a geminivirus.Fundamental research · 2026Article
- Overexpression of the heavy metal-associated isoprenylated plant protein geneHorticulture research · 2026Article
- HIPPs of all trades: insights from pathogens on the function of HMA proteins at plasmodesmata.Journal of experimental botany · 2026Review
- Genome-wide identification and stress response expression analysis of the heavy metal-associated isoprenylated plant protein gene family in tobacco (Nicotiana tabacum L.).BMC plant biology · 2025Article
- Protein Prenylation in Plants: Mechanisms and Functional Implications.Plants (Basel, Switzerland) · 2025Review
- The Magnaporthe oryzae effector Pwl2 alters HIPP43 localization to suppress host immunity.The Plant cell · 2025Article
- Article
- The blast pathogen effector AVR-Pik binds and stabilizes rice heavy metal-associated (HMA) proteins to co-opt their function in immunity.PLoS pathogens · 2024Article
- Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Plants (Basel, Switzerland) · 2024Article
- Article
- Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family fromFrontiers in plant science · 2024Article
- Article
- Overexpression ofInternational journal of molecular sciences · 2023Article
- Heavy Metal-Associated Isoprenylated Plant Proteins (HIPPs) at Plasmodesmata: Exploring the Link between Localization and Function.Plants (Basel, Switzerland) · 2023Review
- An investigation of zinc isotope fractionation in cacao (Theobroma cacao L.) and comparison of zinc and cadmium isotope compositions in hydroponic plant systems under high cadmium stress.Scientific reports · 2023Article
- The Barley Heavy Metal Associated Isoprenylated Plant Protein HvFP1 Is Involved in a Crosstalk between the Leaf Development and Abscisic Acid-Related Drought Stress Responses.Plants (Basel, Switzerland) · 2022Article
- DciA Helicase Operators Exhibit Diversity across Bacterial Phyla.Journal of bacteriology · 2022Article
- Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (International journal of molecular sciences · 2020Article
- Small RNA sequencing identifies cucumber miRNA roles in waterlogging-triggered adventitious root primordia formation.Molecular biology reports · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ion uptake, transport, and sequestration are essential to meet the nutritional requirements for plant growth and development. Furthermore, regulation of these processes is critical for plants to tolerate toxic levels of ions. The examination of isoprenylated proteins encoded by Arabidopsis thaliana and Glycine max cDNAs revealed a unique family of proteins containing putative metal-binding motifs (the core sequence is M/LXCXXC). Here, we describe this new class of proteins, which are capable of being isoprenylated and binding transition metal ions. Members of this family contain consensus isoprenylation (CaaX) sites, which we demonstrate are efficiently isoprenylated in vitro. ATFP3, a representative of the Arabidopsis family, was expressed in Escherichia coli and examined for metal-binding activity in vitro. Analysis of the interaction of ATFP3 with metal-chelating columns (IMAC) suggested that it binds to Cu2+, Ni2+, or Zn2+. To test whether proteins with these characteristics are present in other plant species, tobacco BY2 cells were labeled in vivo with [14C]mevalonate and the resulting mevalonate-labeled proteins were tested for metal-binding activity. Several soluble, isoprenylated proteins which bound copper-IMAC columns were revealed. Consistent with a wide-spread distribution of these proteins in plants, their presence was observed in Arabidopsis, soybean, and tobacco.
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Registered trials
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