ArticlePlant molecular biology1999
A bacterial artificial chromosome library for soybean PI 437654 and identification of clones associated with cyst nematode resistance.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 74 citations in OpenAlex.
- Construction and characterization of a BAC library from the Coffea arabica genotype Timor Hybrid CIFC 832/2.Genetica · 2013Article
- Rj (rj) genes involved in nitrogen-fixing root nodule formation in soybean.Breeding science · 2012Article
- Construction of a llama bacterial artificial chromosome library with approximately 9-fold genome equivalent coverage.Journal of biomedicine & biotechnology · 2012Article
- Expression patterns in soybean resistant to Phakopsora pachyrhizi reveal the importance of peroxidases and lipoxygenases.Functional & integrative genomics · 2008Article
- An overview of the apple genome through BAC end sequence analysis.Plant molecular biology · 2008Article
- Soybean genomics: Developments through the use of cultivar "Forrest".International journal of plant genomics · 2008Article
- Article
- Construction of a quinoa (Chenopodium quinoa Willd.) BAC library and its use in identifying genes encoding seed storage proteins.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Physical mapping and microsynteny of Brassica rapa ssp. pekinensis genome corresponding to a 222 kbp gene-rich region of Arabidopsis chromosome 4 and partially duplicated on chromosome 5.Molecular genetics and genomics : MGG · 2005Article
- Construction and characterization of a peanut HindIII BAC library.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Bacterial artificial chromosome (BAC) library resource for positional cloning of pest and disease resistance genes in cassava (Manihot esculenta Crantz).Plant molecular biology · 2004Article
- Construction and characterization of a soybean bacterial artificial chromosome library and use of multiple complementary libraries for genome physical mapping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Construction of a BAC library of Korean ginseng and initial analysis of BAC-end sequences.Molecular genetics and genomics : MGG · 2004Article
- National Science Foundation-sponsored workshop report. Draft plan for soybean genomics.Plant physiology · 2004Article
- Construction and characterization of a soybean yeast artificial chromosome library and identification of clones for the Rps6 region.Functional & integrative genomics · 2003Article
- BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Characterization of three maize bacterial artificial chromosome libraries toward anchoring of the physical map to the genetic map using high-density bacterial artificial chromosome filter hybridization.Plant physiology · 2002Article
- A gene-enriched BAC library for cloning large allele-specific fragments from maize: isolation of a 240-kb contig of the bronze region.Genome research · 2000Article
- A deep-coverage tomato BAC library and prospects toward development of an STC framework for genome sequencing.Genome research · 2000Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have constructed a soybean bacterial artificial chromosome (BAC) library using the plant introduction (PI) 437654. The library contains 73 728 clones stored in 192 384-well microtiter plates. A random sampling of 230 BACs indicated an average insert size of 136 kb with a range of 20 to 325 kb, and less than 4% of the clones do not contain inserts. Ninety percent of BAC clones in the library have an average insert size greater than 100 kb. Based on a genome size of 1115 Mb, library coverage is 9 haploid genome equivalents. Screening the BAC library colony filters with cpDNA sequences showed that contamination of the genomic library with chloroplast clones was low (1.85%). Library screening with three genomic RFLP probes linked to soybean cyst nematode (SCN) resistance genes resulted in an average of 18 hits per probe (range 7 to 30). Two separate pools of forward and reverse suppression subtractive cDNAs obtained from SCN-infected and uninfected roots of PI437654 were hybridized to the BAC library filters. The 488 BACs identified from positive signals were fingerprinted and analyzed using FPC software (version 4.0) resulting in 85 different contigs. Contigs were grouped and analyzed in three categories: (1) contigs of BAC clones which hybridized to forward subtracted cDNAs, (2) contigs of BAC clones which hybridized to reverse subtracted cDNAs, and (3) contigs of BAC clones which hybridized to both forward and reverse subtracted cDNAs. This protocol provides an estimate of the number of genomic regions involved in early resistance response to a pathogenic attack.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.