ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1999
Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.).
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 89 citations in OpenAlex.
- Advanced environmental scanning electron microscopy reveals natural surface nano-morphology of condensed mitotic chromosomes in their native state.Scientific reports · 2024Article
- Helical coiling of metaphase chromatids.Nucleic acids research · 2023Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Cytomolecular analysis of mutants, breeding lines, and varieties of camelina (Camelina sativa L. Crantz).Journal of applied genetics · 2021Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Dynamic evolution of Panax species.Genes & genomics · 2021Review
- Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes.International journal of molecular sciences · 2021Article
- Proteome Analysis of Condensed Barley Mitotic Chromosomes.Frontiers in plant science · 2021Article
- Article
- A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp.Scientific reports · 2020Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- The Coiled-Coil NLRPlant physiology · 2019Article
- Article
- UNcleProt (Universal Nuclear Protein database of barley): The first nuclear protein database that distinguishes proteins from different phases of the cell cycle.Nucleus (Austin, Tex.) · 2017Article
- Rapid gene isolation in barley and wheat by mutant chromosome sequencing.Genome biology · 2016Article
- Towards development of new ornamental plants: status and progress in wide hybridization.Planta · 2016Review
- Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization.Annals of botany · 2015Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-yield method for isolation of barley chromosomes in suspension, their analysis and sorting using flow cytometry is described. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatment with 2 mmol/L hydroxyurea for 18 h, 2.5 micromol/L amiprophos methyl for 2 h, and ice water (overnight). This treatment resulted in metaphase indices exceeding 50%. Synchronized root tips were fixed in 2% formaldehyde for 20 min and chromosomes were released into a lysis buffer by mechanical homogenization, producing, on average, 5 x 10(5) chromosomes from 50 root tips. The isolated chromosomes were morphologically intact and suitable for flow cytometric analysis and sorting. While it was possible to discriminate and sort only one chromosome from a barley cultivar with standard karyotype, up to three chromosomes could be sorted in translocation lines with morphologically distinct chromosomes. The purity of chromosome fractions, estimated after PRINS with primers specific for GAA microsatellites, reached 97%. PCR with chromosome-specific primers confirmed the purity and suitability of flow-sorted chromosomes for physical mapping of DNA sequences.
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