ArticleThe Plant cell1999
Peroxisomal membrane ascorbate peroxidase is sorted to a membranous network that resembles a subdomain of the endoplasmic reticulum.
Article in The Plant cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 131 citations in OpenAlex.
- Evidence for peroxisomal redundancy among the glucose-6-phosphate dehydrogenase isoforms of Arabidopsis thaliana.Plant & cell physiology · 2025Article
- Ascorbate peroxidase in fruits and modulation of its activity by reactive species.Journal of experimental botany · 2024Review
- Relevance of the Exocyst in ArabidopsisInternational journal of molecular sciences · 2022Article
- Sugar signal mediates flavonoid biosynthesis in tea leaves.Horticulture research · 2022Article
- Natural variation in rice ascorbate peroxidase gene APX9 is associated with a yield-enhancing QTL cluster.Journal of experimental botany · 2021Article
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- Arabidopsis APx-R Is a Plastidial Ascorbate-Independent Peroxidase Regulated by Photomorphogenesis.Antioxidants (Basel, Switzerland) · 2021Article
- The Arabidopsis Plastidial Glucose-6-Phosphate Transporter GPT1 is Dually Targeted to Peroxisomes via the Endoplasmic Reticulum.The Plant cell · 2020Article
- Impairment of peroxisomal APX and CAT activities increases protection of photosynthesis under oxidative stress.Journal of experimental botany · 2019Article
- Dynamics of Peroxisome Homeostasis and Its Role in Stress Response and Signaling in Plants.Frontiers in plant science · 2019Review
- Peroxisome biogenesis, membrane contact sites, and quality control.EMBO reports · 2019Review
- De novo peroxisome biogenesis: Evolving concepts and conundrums.Biochimica et biophysica acta · 2016Review
- Human Peroxin PEX3 Is Co-translationally Integrated into the ER and Exits the ER in Budding Vesicles.Traffic (Copenhagen, Denmark) · 2016Article
- Transcriptome assembly, profiling and differential gene expression analysis of the halophyte Suaeda fruticosa provides insights into salt tolerance.BMC genomics · 2015Article
- Multiple pathways for protein transport to peroxisomes.Journal of molecular biology · 2015Review
- Over-expression of the peroxisomal ascorbate peroxidase (SbpAPX) gene cloned from halophyte Salicornia brachiata confers salt and drought stress tolerance in transgenic tobacco.Marine biotechnology (New York, N.Y.) · 2014Article
- Emerging role of the endoplasmic reticulum in peroxisome biogenesis.Frontiers in physiology · 2013Review
- Tail-anchored PEX26 targets peroxisomes via a PEX19-dependent and TRC40-independent class I pathway.The Journal of cell biology · 2013Article
- Simultaneous live-imaging of peroxisomes and the ER in plant cells suggests contiguity but no luminal continuity between the two organelles.Frontiers in physiology · 2013Article
- Plant peroxisomes: biogenesis and function.The Plant cell · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The peroxisomal isoform of ascorbate peroxidase (APX) is a novel membrane isoform that functions in the regeneration of NAD(+) and protection against toxic reactive oxygen species. The intracellular localization and sorting of peroxisomal APX were examined both in vivo and in vitro. Epitope-tagged peroxisomal APX, which was expressed transiently in tobacco BY-2 cells, localized to a reticular/circular network that resembled endoplasmic reticulum (ER; 3,3'-dihexyloxacarbocyanine iodide-stained membranes) and to peroxisomes. The reticular network did not colocalize with other organelle marker proteins, including three ER reticuloplasmins. However, in vitro, peroxisomal APX inserted post-translationally into the ER but not into other purified organelle membranes (including peroxisomal membranes). Insertion into the ER depended on the presence of molecular chaperones and ATP. These results suggest that regions of the ER serve as a possible intermediate in the sorting pathway of peroxisomal APX. Insight into this hypothesis was obtained from in vivo experiments with brefeldin A (BFA), a toxin that blocks vesicle-mediated protein export from ER. A transiently expressed chloramphenicol acetyltransferase-peroxisomal APX (CAT-pAPX) fusion protein accumulated only in the reticular/circular network in BFA-treated cells; after subsequent removal of BFA from these cells, the CAT-pAPX was distributed to preexisting peroxisomes. Thus, plant peroxisomal APX, a representative enzymatic peroxisomal membrane protein, is sorted to peroxisomes through an indirect pathway involving a preperoxisomal compartment with characteristics of a distinct subdomain of the ER, possibly a peroxisomal ER subdomain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.