ArticleJournal of bacteriology1999
Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 91 citations in OpenAlex.
- Homology-mediated transformation of frog-killing fungusProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploiting bacteria for cancer immunotherapy.Nature reviews. Clinical oncology · 2024Review
- Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation.Microbiology spectrum · 2024Article
- Recombinant AttenuatedCancers · 2022Review
- inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes.Microorganisms · 2022Article
- Bacteria-Mediated Synergistic Cancer Therapy: Small Microbiome Has a Big Hope.Nano-micro letters · 2021Review
- Maximizing the Efficiency of Vanillin Production by Biocatalyst Enhancement and Process Optimization.Frontiers in bioengineering and biotechnology · 2019Article
- Tumour-targeting bacteria engineered to fight cancer.Nature reviews. Cancer · 2018Review
- An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species.AMB Express · 2017Article
- A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies.Scientific reports · 2015Article
- Essential validation methods for E. coli strains created by chromosome engineering.Journal of biological engineering · 2015Article
- Efficient gene delivery into cell lines and stem cells using baculovirus.Nature protocols · 2014Article
- Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci.Microbial cell factories · 2013Article
- Supplementation of substrate uptake gene enhances the expression of rhIFN-β in high cell density fed-batch cultures of Escherichia coli.Molecular biotechnology · 2013Article
- A standard vector for the chromosomal integration and characterization of BioBrick™ parts in Escherichia coli.Journal of biological engineering · 2013Article
- Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2'-fucosyllactose.Microbial cell factories · 2013Article
- A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination.BMC microbiology · 2013Article
- A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli.Microbial cell factories · 2012Article
- One-pot bio-synthesis: N-acetyl-D-neuraminic acid production by a powerful engineered whole-cell catalyst.Scientific reports · 2011Article
- Metabolic engineering for production of biorenewable fuels and chemicals: contributions of synthetic biology.Journal of biomedicine & biotechnology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of vectors which facilitates the sequential integration of new functions into the Escherichia coli chromosome by homologous recombination has been developed. These vectors are based on plasmids described by Posfai et al. (J. Bacteriol. 179:4426-4428, 1997) which contain conditional replicons (pSC101 or R6K), a choice of three selectable markers (ampicillin, chloramphenicol, or kanamycin), and a single FRT site. The modified vectors contain two FRT sites which bracket a modified multiple cloning region for DNA insertion. After integration, a helper plasmid expressing the flippase (FLP) recombinase allows precise in vivo excision of the replicon and the marker used for selection. Sites are also available for temporary insertion of additional functions which can be subsequently deleted with the replicon. Only the DNA inserted into the multiple cloning sites (passenger genes and homologous fragment for targeting) and a single FRT site (68 bp) remain in the chromosome after excision. The utility of these vectors was demonstrated by integrating Zymomonas mobilis genes encoding the ethanol pathway behind the native chromosomal adhE gene in strains of E. coli K-12 and E. coli B. With these vectors, a single antibiotic selection system can be used repeatedly for the successive improvement of E. coli strains with precise deletion of extraneous genes used during construction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.