ArticleJournal of bacteriology1999
Molecular cloning and characterization of the Helicobacter pylori fliD gene, an essential factor in flagellar structure and motility.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Identification of biofilm-associated genes inBiofilm · 2026Article
- Computational design of a multi-epitope vaccine against Helicobacter pylori targeting CagA, FliD, urease, and OipA with cholera toxin B subunit (CTB) adjuvant.BMC microbiology · 2026Article
- Dissecting the role of flagellar subunits inJournal of bacteriology · 2025Article
- Research advances of the establishment and characterization ofFrontiers in microbiology · 2025Review
- Changes in potential pathogenicity-associated proteins ofFrontiers in microbiology · 2025Article
- Review
- Motility of Different GastricMicroorganisms · 2023Review
- How Long Will It Take to Launch an EffectiveInfection and drug resistance · 2023Review
- Comparative genomics analysis of statistically significant genomic islands of Helicobacter pylori strains for better understanding the disease prognosis.Bioscience reports · 2022Article
- A plasmid-freeFrontiers in bioengineering and biotechnology · 2022Article
- Pathogenesis and clinical management ofWorld journal of gastroenterology · 2019Review
- Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi.Molecular microbiology · 2019Article
- Article
- Role of Flagella in the Pathogenesis of Helicobacter pylori.Current microbiology · 2017Review
- Strategies used byWorld journal of gastroenterology · 2017Review
- Article
- Article
- Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli.Infection and immunity · 2016Article
- Helicobacter pylori infection: An overview of bacterial virulence factors and pathogenesis.Biomedical journal · 2016Review
- Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. Flagellar motility is regarded as a major factor in the colonizing ability of H. pylori. The functional roles of flagellar structural proteins other than FlaA, FlaB, and FlgE are not well understood. The fliD operon of H. pylori consists of flaG, fliD, and fliS genes, in the order stated, under the control of a sigma(28)-dependent promoter. In an effort to elucidate the function of the FliD protein, a hook-associated protein 2 homologue, in flagellar morphogenesis and motility, the fliD gene (2,058 bp) was cloned and isogenic mutants were constructed by disruption of the fliD gene with a kanamycin resistance cassette and electroporation-mediated allelic-exchange mutagenesis. In the fliD mutant, morphologically abnormal flagellar appendages in which very little filament elongation was apparent were observed. The fliD mutant strain was completely nonmotile, indicating that these abnormal flagella were functionally defective. Furthermore, the isogenic fliD mutant of H. pylori SS1, a mouse-adapted strain, was not able to colonize the gastric mucosae of host mice. These results suggest that H. pylori FliD is an essential element in the assembly of the functional flagella that are required for colonization of the gastric mucosa.
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