ArticleProceedings of the National Academy of Sciences of the United States of America1999
A small asparagine-rich protein required for S-allele-specific pollen rejection in Nicotiana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 180 citations in OpenAlex.
- The genetic architecture of quantitative variation in the self-incompatibility response within Phlox drummondii (Polemoniaceae).Genetics · 2025Article
- S-RNase-based self-incompatibility in angiosperms: Degradation, condensation, and evolution.Plant physiology · 2025Review
- Molecular insights into self-incompatibility systems: From evolution to breeding.Plant communications · 2024Review
- Article
- Genetic control of compatibility in crosses between wheat and its wild or cultivated relatives.Plant biotechnology journal · 2022Review
- A pair of non-Mendelian genes at the Ga2 locus confer unilateral cross-incompatibility in maize.Nature communications · 2022Article
- Gene coexpression analysis reveals key pathways and hub genes related to late-acting self-incompatibility inFrontiers in plant science · 2022Article
- A Cysteine-Rich Protein, SpDIR1L, Implicated in S-RNase-Independent Pollen Rejection in the Tomato (International journal of molecular sciences · 2021Article
- Migration through a Major Andean Ecogeographic Disruption as a Driver of Genetic and Phenotypic Diversity in a Wild Tomato Species.Molecular biology and evolution · 2021Article
- Ornithine decarboxylase genes contribute to S-RNase-independent pollen rejection.Plant physiology · 2021Article
- Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.PloS one · 2021Article
- Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology.Frontiers in plant science · 2020Review
- Overcoming Self-Incompatibility in Diploid Potato Using CRISPR-Cas9.Frontiers in plant science · 2019Article
- Finding a Compatible Partner: Self-Incompatibility in European Pear (Frontiers in plant science · 2019Review
- SIPP, a Novel Mitochondrial Phosphate Carrier, Mediates in Self-Incompatibility.Plant physiology · 2017Article
- Two Loci Contribute Epistastically to Heterospecific Pollen Rejection, a Postmating Isolating Barrier Between Species.G3 (Bethesda, Md.) · 2017Article
- Transcriptomic analysis links gene expression to unilateral pollen-pistil reproductive barriers.BMC plant biology · 2017Article
- Pollen-Pistil Interactions and Their Role in Mate Selection.Plant physiology · 2017Review
- Unilateral incompatibility gene ui1.1 encodes an S-locus F-box protein expressed in pollen of Solanum species.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- In vitro inhibition of incompatible pollen tubes in Nicotiana alata involves the uncoupling of the F-actin cytoskeleton and the endomembrane trafficking system.Protoplasma · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although S-locus RNases (S-RNases) determine the specificity of pollen rejection in self-incompatible (SI) solanaceous plants, they alone are not sufficient to cause S-allele-specific pollen rejection. To identify non-S-RNase sequences that are required for pollen rejection, a Nicotiana alata cDNA library was screened by differential hybridization. One clone, designated HT, hybridized strongly to RNA from N. alata styles but not to RNA from Nicotiana plumbaginifolia, a species known to lack one or more factors necessary for S-allele-specific pollen rejection. Sequence analysis revealed a 101-residue ORF including a putative secretion signal and an asparagine-rich domain near the C terminus. RNA blot analysis showed that the HT-transcript accumulates in the stigma and style before anthesis. The timing of HT-expression lags slightly behind S(C10)-RNase in SI N. alata S(C10)S(C10) and is well correlated with the onset of S-allele-specific pollen rejection in the style. An antisense-HT construct was prepared to test for a role in pollen rejection. Transformed (N. plumbaginifolia x SI N. alata S(C10)S(C10)) hybrids with reduced levels of HT-protein continued to express S(C10)-RNase but failed to reject S(C10)-pollen. Control hybrids expressing both S(C10)-RNase and HT-protein showed a normal S-allele-specific pollen rejection response. We conclude that HT-protein is directly implicated in pollen rejection.
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