ArticlePlant physiology1999
Magnesium Sensitizes Slow Vacuolar Channels to Physiological Cytosolic Calcium and Inhibits Fast Vacuolar Channels in Fava Bean Guard Cell Vacuoles.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 102 citations in OpenAlex.
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- TPC1-Type Channels inPlants (Basel, Switzerland) · 2022Article
- Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles.Stress biology · 2022Review
- Major vacuolar TPC1 channel in stress signaling: what matters, KStress biology · 2022Review
- Voltage-gating and cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.International journal of molecular sciences · 2021Article
- MgInternational journal of molecular sciences · 2020Article
- The Membrane Transport System of the Guard Cell and Its Integration for Stomatal Dynamics.Plant physiology · 2017Review
- The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix.Cellular and molecular life sciences : CMLS · 2016Article
- Article
- The phosphoinositide PI(3,5)P₂ mediates activation of mammalian but not plant TPC proteins: functional expression of endolysosomal channels in yeast and plant cells.Cellular and molecular life sciences : CMLS · 2014Article
- Polyamines control of cation transport across plant membranes: implications for ion homeostasis and abiotic stress signaling.Frontiers in plant science · 2014Review
- Calcium Signals from the Vacuole.Plants (Basel, Switzerland) · 2013Review
- Cation-permeable vacuolar ion channels in the moss Physcomitrella patens: a patch-clamp study.Planta · 2013Article
- Modulation of plant TPC channels by polyunsaturated fatty acids.Journal of experimental botany · 2012Article
- Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus-response analyses.Annals of botany · 2012Review
- The putative K(+) channel subunit AtKCO3 forms stable dimers in Arabidopsis.Frontiers in plant science · 2012Article
- Plant ion channels: gene families, physiology, and functional genomics analyses.Annual review of physiology · 2009Review
- The Clickable Guard Cell, Version II: Interactive Model of Guard Cell Signal Transduction Mechanisms and Pathways.The arabidopsis book · 2008Article
- Homeostatic control of slow vacuolar channels by luminal cations and evaluation of the channel-mediated tonoplast Ca2+ fluxes in situ.Journal of experimental botany · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuolar ion channels in guard cells play important roles during stomatal movement and are regulated by many factors including Ca(2+), calmodulin, protein kinases, and phosphatases. We report that physiological cytosolic and luminal Mg(2+) levels strongly regulate vacuolar ion channels in fava bean (Vicia faba) guard cells. Luminal Mg(2+) inhibited fast vacuolar (FV) currents with a K(i) of approximately 0.23 mM in a voltage-dependent manner at positive potentials on the cytoplasmic side. Cytosolic Mg(2+) at 1 mM also inhibited FV currents. Furthermore, in the absence of cytosolic Mg(2+), cytosolic Ca(2+) at less than 10 µM did not activate slow vacuolar (SV) currents. However, when cytosolic Mg(2+) was present, submicromolar concentrations of cytosolic Ca(2+) activated SV currents with a K(d) of approximately 227 nM, suggesting a synergistic Mg(2+)-Ca(2+) effect. The activation potential of SV currents was shifted toward physiological potentials in the presence of cytosolic Mg(2+) concentrations. The direction of SV currents could also be changed from outward to both outward and inward currents. Our data predict a model for SV channel regulation, including a cytosolic binding site for Ca(2+) with an affinity in the submicromolar range and a cytosolic low-affinity Mg(2+)-Ca(2+) binding site. SV channels are predicted to contain a third binding site on the vacuolar luminal side, which binds Ca(2+) and is inhibitory. In conclusion, cytosolic Mg(2+) sensitizes SV channels to physiological cytosolic Ca(2+) elevations. Furthermore, we propose that cytosolic and vacuolar Mg(2+) concentrations ensure that FV channels do not function as a continuous vacuolar K(+) leak, which would prohibit stomatal opening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.