ArticlePlant physiology1999
Peroxisomal NADP-Dependent Isocitrate Dehydrogenase. Characterization and Activity Regulation during Natural Senescence.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 151 citations in OpenAlex.
- Spatiotemporal Dynamics of Cytosolic NADPH in Living Arabidopsis thaliana.Plant, cell & environment · 2026Article
- Circular and Long Non-Coding RNAs in Cancer Metabolism: Dual Perspective of Biomarkers and Therapeutic Targets.Non-coding RNA · 2026Review
- Unraveling the persistent renal impact of intrauterine growth restriction and catch-up growth: integrating morphological insights with metabolomic profiling.Journal of molecular histology · 2025Article
- Characteristics and Prognosis of "Acute Promyelocytic Leukemia-like" Nucleophosmin-1-Mutated Acute Myeloid Leukemia in a Retrospective Patient Cohort.Biomedicines · 2024Article
- Distribution and Functional Analysis of Isocitrate Dehydrogenases across Kinetoplastids.Genome biology and evolution · 2024Article
- Pepper Fruit Extracts Show Anti-Proliferative Activity against Tumor Cells Altering Their NADPH-Generating Dehydrogenase and Catalase Profiles.Antioxidants (Basel, Switzerland) · 2023Article
- Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth.Frontiers in plant science · 2023Article
- Deficiency of a peroxisomal NADP-isocitrate dehydrogenase leads to dwarf plant and defect seed in upland cotton.Frontiers in plant science · 2022Article
- Peroxisomal Proteome Mining of Sweet Pepper (Frontiers in plant science · 2022Article
- Phytochemicals: "A Small Defensive Advantage for Plants and Fungi; a Great Remedy for the Health of Mankind".Molecules (Basel, Switzerland) · 2021Article
- Dietary N-carbamylglutamate or l-arginine supplementation improves hepatic energy status and mitochondrial function and inhibits the AMP-activated protein kinase-peroxisome proliferator-activated receptor γ coactivator-1α-transcription factor A pathway in intrauterine-growth-retarded suckling lambs.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Are Ancestral Medical Practices the Future Solution to Today's Medical Problems?Molecules (Basel, Switzerland) · 2021Article
- Peroxisomes as redox-signaling nodes in intracellular communication and stress responses.Plant physiology · 2021Review
- Nitric Oxide (NO) Scaffolds the Peroxisomal Protein-Protein Interaction Network in Higher Plants.International journal of molecular sciences · 2021Review
- Tricarboxylic Acid (TCA) Cycle Intermediates: Regulators of Immune Responses.Life (Basel, Switzerland) · 2021Review
- Auxin is involved in arbuscular mycorrhizal fungi-promoted tomato growth and NADP-malic enzymes expression in continuous cropping substrates.BMC plant biology · 2021Article
- Characterization of NADFrontiers in nutrition · 2021Article
- Metformin as Potential Therapy for High-Grade Glioma.Cancers · 2020Article
- Targeted Therapies in Cholangiocarcinoma: Emerging Evidence from Clinical Trials.Medicina (Kaunas, Lithuania) · 2019Review
- Cytosolic Isocitrate Dehydrogenase fromAntioxidants (Basel, Switzerland) · 2019Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The peroxisomal localization and characterization of NADP-dependent isocitrate dehydrogenase (perICDH) in young and senescent pea (Pisum sativum) leaves was studied by subcellular fractionation, kinetic analysis, immunoblotting, and immunoelectron microscopy. The subunit molecular mass for perICDH determined by immunoblotting was 46 kD. By isoelectric focusing (IEF) of the peroxisomal matrix fraction, the NADP-ICDH activity was resolved into four isoforms, perICDH-1 to perICDH-4, with isoelectric points (pIs) of 6.0, 5.6, 5.4, and 5.2, respectively. The kinetic properties of the NADP-ICDH in peroxisomes from young and senescent pea leaves were analyzed. The maximum initial velocity was the same in peroxisomes from young and senescent leaves, while the Michaelis constant value in senescent leaf peroxisomes was 11-fold lower than in young leaf peroxisomes. The protein levels of NADP-ICDH in peroxisomes were not altered during senescence. The kinetic behavior of this enzyme suggests a possible fine control of enzymatic activity by modulation of its Michaelis constant during the natural senescence of pea leaves. After embedding, electron microscopy immunogold labeling of NADP-ICDH confirmed that this enzyme was localized in the peroxisomal matrix. Peroxisomal NADP-ICDH represents an alternative dehydrogenase in these cell organelles and may be the main system for the reduction of NADP to NADPH for its re-utilization in the peroxisomal metabolism.
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Registered trials
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