ArticlePlant physiology1999
Sucrose and Cytokinin Modulation of WPK4, a Gene Encoding a SNF1-Related Protein Kinase from Wheat.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Transcriptomic analysis of the defense response in "Cabernet Sauvignon" grape leaf induced byPlant direct · 2024Article
- Sucrose nonfermenting-1-related protein kinase 1 regulates sheath-to-panicle transport of nonstructural carbohydrates during rice grain filling.Plant physiology · 2022Article
- Sugar metabolism during pre- and post-fertilization events in plants under high temperature stress.Plant cell reports · 2022Review
- Article
- Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi.Scientific reports · 2021Article
- The Sugar-Signaling Hub: Overview of Regulators and Interaction with the Hormonal and Metabolic Network.International journal of molecular sciences · 2018Review
- The wheat TabZIP2 transcription factor is activated by the nutrient starvation-responsive SnRK3/CIPK protein kinase.Plant molecular biology · 2018Article
- Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase Is Phosphorylated during Seed Development.Frontiers in plant science · 2017Article
- TaCIPK29, a CBL-interacting protein kinase gene from wheat, confers salt stress tolerance in transgenic tobacco.PloS one · 2013Article
- SnRK2 protein kinases--key regulators of plant response to abiotic stresses.Omics : a journal of integrative biology · 2011Review
- Nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase is phosphorylated in wheat endosperm at serine-404 by an SNF1-related protein kinase allosterically inhibited by ribose-5-phosphate.Plant physiology · 2011Article
- Sucrose non-fermenting 1-related protein kinase 2 (SnRK2): a family of protein kinases involved in hyperosmotic stress signaling.Physiology and molecular biology of plants : an international journal of functional plant biology · 2008Article
- Large scale transcriptome analysis of the effects of nitrogen nutrition on accumulation of stem carbohydrate reserves in reproductive stage wheat.Plant molecular biology · 2008Article
- Arabidopsis cytokinin-resistant mutant, cnr1, displays altered auxin responses and sugar sensitivity.Plant molecular biology · 2006Article
- Biochemical characterization of the tobacco 42-kD protein kinase activated by osmotic stress.Plant physiology · 2004Article
- Isolation and characterization of a novel rice Ca2+-regulated protein kinase gene involved in responses to diverse signals including cold, light, cytokinins, sugars and salts.Plant molecular biology · 2003Article
- Comparative transcriptional profiling of placenta and endosperm in developing maize kernels in response to water deficit.Plant physiology · 2003Article
- The CRK1 receptor-like kinase gene of tobacco is negatively regulated by cytokinin.Plant molecular biology · 2002Article
- The cell biology of phytochrome signalling.The New phytologist · 2002Review
- Expression, activation, and biochemical properties of a novel Arabidopsis protein kinase.Plant physiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
WPK4, a gene encoding a putative protein kinase, was initially identified in wheat (Triticum aestivum) and shown to be up-regulated by light, nutrient deprivation, and cytokinins. To confirm that WPK4 has protein kinase activity, the protein was produced in Escherichia coli as a fusion protein with glutathione S-transferase. The purified protein exhibited autophosphorylation activity and phosphorylated both myelin basic protein and a peptide fragment of rice 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Levels of WPK4 transcripts in wheat seedlings were increased and decreased by the removal and addition of sucrose (Suc), respectively, to the culture medium. The introduction of the N-terminal kinase region of WPK4 into the yeast snf1 mutant cells, which cannot utilize Suc as a carbon source, rescued growth in Suc-containing medium. Cytokinins up-regulated the accumulation of WPK4 transcripts, but their effects were cancelled by the addition of Suc. Our results suggest that Suc negatively regulates the signaling pathway in which transcriptional activation of WPK4 is mediated by cytokinins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.