ArticlePlant physiology1999
Cloning and first functional characterization of a plant cyclic nucleotide-gated cation channel.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 275 citations in OpenAlex.
- Review
- Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity.Nature plants · 2025Article
- Article
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- Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.Bioscience reports · 2024Article
- Sulfated peptides and their receptors: Key regulators of plant development and stress adaptation.Plant communications · 2024Review
- Plant adenylate cyclases have come full circle.Nature plants · 2023Review
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- Pathogen-induced autophagy regulates monolignol transport and lignin formation in plant immunity.Autophagy · 2023Article
- Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomatal closure in Arabidopsis.The Plant cell · 2023Article
- A glutamate receptor-like gene is involved in ABA-mediated growth control inPlant signaling & behavior · 2022Article
- Single-cell transcriptome sequencing atlas of cassava tuberous root.Frontiers in plant science · 2022Article
- Deciphering the Role of Ion Channels in Early Defense Signaling against Herbivorous Insects.Cells · 2021Review
- The Complex Story of Plant Cyclic Nucleotide-Gated Channels.International journal of molecular sciences · 2021Review
- Potential Networks of Nitrogen-Phosphorus-Potassium Channels and Transporters in Arabidopsis Roots at a Single Cell Resolution.Frontiers in plant science · 2021Article
- Cross Talk Between Cyclic Nucleotides and Calcium Signaling Pathways in Plants-Achievements and Prospects.Frontiers in plant science · 2021Review
- Plant Cyclic Nucleotide-Gated Channels: New Insights on Their Functions and Regulation.Plant physiology · 2020Article
- Cyclic AMP: A Polyhedral Signalling Molecule in Plants.International journal of molecular sciences · 2020Review
- Suppression of class I compensated cell enlargement by xs2 mutation is mediated by salicylic acid signaling.PLoS genetics · 2020Article
- MgInternational journal of molecular sciences · 2020Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclic nucleotide-gated (cng) non-selective cation channels have been cloned from a number of animal systems. These channels are characterized by direct gating upon cAMP or cGMP binding to the intracellular portion of the channel protein, which leads to an increase in channel conductance. Animal cng channels are involved in signal transduction systems; they translate stimulus-induced changes in cytosolic cyclic nucleotide into altered cell membrane potential and/or cation flux as part of a signal cascade pathway. Putative plant homologs of animal cng channels have been identified. However, functional characterization (i.e. demonstration of cyclic-nucleotide-dependent ion currents) of a plant cng channel has not yet been accomplished. We report the cloning and first functional characterization of a plant member of this family of ion channels. The Arabidopsis cDNA AtCNGC2 encodes a polypeptide with deduced homology to the alpha-subunit of animal channels, and facilitates cyclic nucleotide-dependent cation currents upon expression in a number of heterologous systems. AtCNGC2 expression in a yeast mutant lacking a low-affinity K(+) uptake system complements growth inhibition only when lipophilic cyclic nucleotides are present in the culture medium. Voltage clamp analysis indicates that Xenopus laevis oocytes injected with AtCNGC2 cRNA demonstrate cyclic-nucleotide-dependent, inward-rectifying K(+) currents. Human embryonic kidney cells (HEK293) transfected with AtCNGC2 cDNA demonstrate increased permeability to Ca(2+) only in the presence of lipophilic cyclic nucleotides. The evidence presented here supports the functional classification of AtCNGC2 as a cyclic-nucleotide-gated cation channel, and presents the first direct evidence (to our knowledge) identifying a plant member of this ion channel family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.