ArticleGenes & development1999
Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
79 citing papers in PubMed, 146 citations in OpenAlex.
- Cryo-electron microscopy structure of the budding yeast telomerase holoenzyme.Science (New York, N.Y.) · 2026Article
- Inverse-Folding Design of Yeast Telomerase RNA Increases Activity In Vitro.Non-coding RNA · 2023Article
- Inverse-folding design of yeast telomerase RNA increases activitybioRxiv : the preprint server for biology · 2023Article
- Many Functions of Telomerase Components: Certainties, Doubts, and Inconsistencies.International journal of molecular sciences · 2022Review
- Repositioning the Sm-Binding Site inNon-coding RNA · 2020Article
- Single-cell imaging reveals unexpected heterogeneity of telomerase reverse transcriptase expression across human cancer cell lines.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Telomerase Mechanism of Telomere Synthesis.Annual review of biochemistry · 2017Review
- The protein subunit of telomerase displays patterns of dynamic evolution and conservation across different metazoan taxa.BMC evolutionary biology · 2017Article
- Analysis of Yeast Telomerase by Primer Extension Assays.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Article
- The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae.eLife · 2015Article
- A second essential function of the Est1-binding arm of yeast telomerase RNA.RNA (New York, N.Y.) · 2015Article
- RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP.The EMBO journal · 2013Article
- A motif in the vertebrate telomerase N-terminal linker of TERT contributes to RNA binding and telomerase activity and processivity.Structure (London, England : 1993) · 2013Article
- A new telomerase RNA element that is critical for telomere elongation.Nucleic acids research · 2013Article
- Article
- The anaphase promoting complex contributes to the degradation of the S. cerevisiae telomerase recruitment subunit Est1p.PloS one · 2013Article
- Suppressing tumor growth of nasopharyngeal carcinoma by hTERTC27 polypeptide delivered through adeno-associated virus plus adenovirus vector cocktail.Chinese journal of cancer · 2012Article
- Stiffened yeast telomerase RNA supports RNP function in vitro and in vivo.RNA (New York, N.Y.) · 2012Article
- Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end.Genetics · 2012Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomerase is a ribonucleoprotein complex that adds telomeric DNA repeats to the ends of most eukaryotic chromosomes. The reverse transcriptase subunit of telomerase (TERT) differs from retroviral reverse transcriptases in having a long basic amino-terminal extension. We made a large library containing random mutations in the amino terminus of the EST2 gene, which encodes the Saccharomyces cerevisiae TERT, and selected functional alleles by their ability to rescue senescence of telomerase-negative cells. Through analysis of 265 mutations, the amino terminus of Est2p was found to contain at least four essential regions. This domain structure was verified by a combination of deletion and alanine-block mutations. Mutations within two essential domains of the protein reduced RNA binding, suggesting that the amino terminus of Est2p makes important contacts with the intrinsic RNA component of telomerase. A mutant close to the amino terminus retained RNA binding and in vitro enzymatic activity but was defective in vivo, suggesting a role in interaction with other macromolecular components of telomerase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.