ArticleBritish journal of pharmacology1999
A major role for the rho-associated coiled coil forming protein kinase in G-protein-mediated Ca2+ sensitization through inhibition of myosin phosphatase in rabbit trachea.
Article in British journal of pharmacology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 109 citations in OpenAlex.
- Review
- GRespiratory research · 2024Review
- Deconstructing aqueous humor outflow - The last 50 years.Experimental eye research · 2020Review
- The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction.eLife · 2020Article
- Article
- Bronchoprotection and bronchorelaxation in asthma: New targets, and new ways to target the old ones.Pharmacology & therapeutics · 2016Review
- Elevated plasma aldosterone is an independent risk factor for erectile dysfunction in men.World journal of urology · 2016Article
- ROCK insufficiency attenuates ozone-induced airway hyperresponsiveness in mice.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- A novel role for RhoA GTPase in the regulation of airway smooth muscle contraction.Canadian journal of physiology and pharmacology · 2015Review
- Article
- ZIPK is critical for the motility and contractility of VSMCs through the regulation of nonmuscle myosin II isoforms.American journal of physiology. Heart and circulatory physiology · 2014Article
- Rho-associated coiled-coil containing kinases (ROCK): structure, regulation, and functions.Small GTPases · 2014Review
- Comparisons of actin filament disruptors and Rho kinase inhibitors as potential antiglaucoma medications.Expert review of ophthalmology · 2012Article
- RGD-Dependent Epithelial Cell-Matrix Interactions in the Human Intestinal Crypt.Journal of signal transduction · 2012Article
- Rho-Rho kinase pathway in the actomyosin contraction and cell-matrix adhesion in immortalized human trabecular meshwork cells.Molecular vision · 2011Article
- Myosin IIb activity and phosphorylation status determines dendritic spine and post-synaptic density morphology.PloS one · 2011Article
- The effects of the small GTPase RhoA on the muscarinic contraction of airway smooth muscle result from its role in regulating actin polymerization.American journal of physiology. Cell physiology · 2010Article
- The washout phenomenon in aqueous outflow--why does it matter?Experimental eye research · 2009Review
- Effects of amitriptyline, a tricyclic antidepressant, on smooth muscle reactivity in isolated rat trachea.Journal of anesthesia · 2009Article
- High concentrations of landiolol, a beta(1)-adrenoceptor antagonist, stimulate smooth muscle contraction of the rat trachea through the Rho-kinase pathway.Journal of anesthesia · 2008Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1 G protein-mediated Ca2+ sensitization of airway smooth muscle contraction was investigated with respect to the relative importance of Rho-associated coiled coil forming protein kinase (ROCK) and protein kinase C (PKC). We examined the effects of Y-27632, a ROCK inhibitor, and GF 109203X, a PKC inhibitor, on guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS)-induced contraction in alpha-toxin- or beta-escin-permeabilized rabbit trachea. 2 Although pre-treatment with Y-27632 dose-dependently inhibited GTPgammaS (10 microM)-induced Ca2+ sensitization of alpha-toxin-permeabilized trachea, a Y-27632-insensitive component (approximately 16% of the maximum contraction) was retained during the early phase of the GTPgammaS response in the presence of Y-27632 (100 microM). 3 GF 109203X (5 microM) abolished 1 microM 4beta-phorbol 12, 13-dibutyrate (PDBu)-induced, but only partially inhibited the GTPgammaS-induced Ca2+ sensitization. A combination of Y-27632 (100 microM) and GF 109203X (5 microM) totally abolished the GTPgammaS response. 4 GTPgammaS caused only a small contraction in the absence of Ca2+. Wortmannin (30 microM), a myosin light chain kinase (MLCK) inhibitor, completely inhibited Ca2+-induced contraction. ATP-triggered contraction of the strip which had been treated with calyculin A (1 microM), a phosphatase inhibitor, in rigor solutions was markedly slowed by worthmannin (30 microM), but not by Y-27632 (100 microM), in the presence of GTPgammaS and Ca2+. 5 GTPgammaS, but not PDBu, contracted the beta-escin-permeabilized trachea in the absence of Ca2+, but the presence of Ca2+-independent MLCK. 6 We conclude that ROCK plays a primary role in G-protein-mediated Ca2+ sensitization, which requires MLCK activity, with minor contribution of PKC to the early phase of contraction, and PDBu utilizes conventional PKC(s) in airway smooth muscle.
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