ArticleBritish journal of cancer1999
Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes.
Article in British journal of cancer, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 156 citations in OpenAlex.
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- Clinical application of circulating tumor cells.Medizinische Genetik : Mitteilungsblatt des Berufsverbandes Medizinische Genetik e.V · 2023Article
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- The potential for liquid biopsies in the precision medical treatment of breast cancer.Cancer biology & medicine · 2016Article
- Evaluating blood levels of neuron specific enolase, chromogranin A, and circulating tumor cells as Merkel cell carcinoma biomarkers.Oncotarget · 2015Article
- Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies.BMC biotechnology · 2010Article
- CpG-island methylation of the ER promoter in colorectal cancer: analysis of micrometastases in lymph nodes from UICC stage I and II patients.British journal of cancer · 2009Article
- Occult disseminated tumor cells in lymph nodes of patients with gastric carcinoma. A critical appraisal of assessment and relevance.Langenbeck's archives of surgery · 2009Article
- Quantitation of circulating tumor cells in blood samples from ovarian and prostate cancer patients using tumor-specific fluorescent ligands.International journal of cancer · 2008Article
- Occult tumor cells in lymph nodes as a predictor for tumor relapse in pancreatic adenocarcinoma.Langenbeck's archives of surgery · 2008Article
- Circulating tumor cells in solid cancer: tumor marker of clinical relevance?Current oncology reports · 2008Review
- Carcinoembryonic antigen and cytokeratin 20 in peritoneal cells of cancer patients: are we aware of what we are detecting by mRNA examination?British journal of cancer · 2008Article
- Morphological and immunohistochemical characterization of isolated tumor cells by p53 status in gastrointestinal tumors.Langenbeck's archives of surgery · 2008Article
- Correlation of CK-20-positive cells in peripheral venous blood with serum CEA levels in patients with colorectal carcinoma.World journal of surgery · 2007Article
- Detection of disseminated tumor cells in the lymph nodes of colorectal cancer patients using a real-time polymerase chain reaction assay.Langenbeck's archives of surgery · 2007Article
- Quantitative real-time RT-PCR of disseminated tumor cells in combination with immunomagnetic cell enrichment.Molecular biotechnology · 2006Article
- Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients.Journal of Zhejiang University. Science. B · 2006Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reverse transcription polymerase chain reaction (RT-PCR) amplification of cytokeratin 20 (CK20) mRNA is considered a promising candidate method for the detection of circulating tumour cells in bone marrow and peripheral blood of cancer patients. In this study we have investigated the diagnostic specificity of the CK20 mRNA detection in samples from healthy donors (HD; n = 33), intensive care units patients (ICU; n = 20) and bone marrow obtained from patients suffering from chronic inflammatory diseases (CID; n = 14). RNAs purified from stabilized lysates showed positive results in 24% of the HD group (8/33), 35% of the ICU group (8/20) and in 40% of the CID group (5/14). The use of Ficoll gradients to separate nucleated cells completely restored the specificity of this CK20 RT-PCR assay. The CK20-expressing cells are positively identified to belong to the granulocyte fraction of leucocytes, which appear to express the gene on a background level. Our results demonstrate for the first time that CK20 mRNA expression is not limited to epithelium. Its occurrence in normal granulocytes has to be considered in tests designed to detect circulating cancer cells or micrometastases.
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