ArticleJournal of muscle research and cell motility1999
Fast calcium removal during single twitches in amphibian skeletal muscle fibres.
Article in Journal of muscle research and cell motility, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Modeling CaFrontiers in physiology · 2016Article
- Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach.Journal of muscle research and cell motility · 2014Article
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Analysis of spontaneous and nerve-evoked calcium transients in intact extraocular muscles in vitro.Experimental eye research · 2012Article
- Kinetic changes in tetanic Ca²⁺ transients in enzymatically dissociated muscle fibres under repetitive stimulation.The Journal of physiology · 2011Article
- Effects of membrane depolarization and changes in extracellular [K(+)] on the Ca (2+) transients of fast skeletal muscle fibers. Implications for muscle fatigue.Journal of muscle research and cell motility · 2010Article
- Luminal Ca(2+) content regulates intracellular Ca(2+) release in subepicardial myocytes of intact beating mouse hearts: effect of exogenous buffers.American journal of physiology. Heart and circulatory physiology · 2010Article
- Myosin heavy chain isoform composition and Ca(2+) transients in fibres from enzymatically dissociated murine soleus and extensor digitorum longus muscles.The Journal of physiology · 2010Article
- The use of CalciumOrange-5N as a specific marker of mitochondrial Ca2+ in mouse skeletal muscle fibers.Pflugers Archiv : European journal of physiology · 2008Article
- Effect of mitochondria poisoning by FCCP on Ca2+ signaling in mouse skeletal muscle fibers.Pflugers Archiv : European journal of physiology · 2008Article
- Control of intracellular calcium in the presence of nitric oxide donors in isolated skeletal muscle fibres from mouse.The Journal of physiology · 2004Article
- Calcium release in skeletal muscle: from K+ contractures to Ca2+ sparks.Journal of muscle research and cell motility · 2001Review
- Spatial Ca(2+) distribution in contracting skeletal and cardiac muscle cells.Biophysical journal · 2000Article
- Fast calcium removal during single twitches in amphibian skeletal muscle fibres.Journal of muscle research and cell motility · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence signals from the calcium sensitive dyes Fluo-3 or Rhod-2 were obtained simultaneously with isometric tension in single fibres isolated from the anterior tibialis muscle of Leptodactylus insularis (20-22 degrees C). Fluo-3 fluorescence signals were transformed into [Ca2+]i transients as previously described. Most of the decay phase of single twitch transient is well fitted by a single exponential (tau of about 10 ms), followed by a slower declining component lasting tens of milliseconds. During short periods, 10 to 20 s, of low frequency stimulation, between 0.2 and 5 Hz, the basal [Ca2+]i increased slowly from 0.1 to about 0.4 microM, with only minor changes in the exponentially decaying phase. In fibres poisoned with thapsigargin or cyclopiazonic acid (1-2 microM) the tau of decay of fluorescence or Ca2+ transients of single twitches was very similar to that observed in non-poisoned fibres. Nevertheless, in poisoned fibres challenged with repetitive stimulation. the tau of Ca2+ transients decay increased from about 10 ms to >40 ms, while the basal [Ca2+]i increased from 0.1 to 2 microM. Short rest periods (about 5 min) could reverse these effects, indicating that they were not a direct consequence of SR Ca 2+ -ATPase inhibition. The correlation coefficient between tau of decay and basal [Ca2+]i was >0.8 (P<0.0001). Qualitatively similar results were obtained measuring Rhod-2 fluorescence signals. A lumped, two-compartment model could account for these results. Loading the fibres with EGTA-AM, diminished the effects of prolonged stimulation observed in poisoned fibres. Moreover, we show that the Na+ - Ca2+ exchange mechanism does not participate appreciably in fast Ca2+ removal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.