ArticleThe Biochemical journal1999
Competition between Escherichia coli strains expressing either a periplasmic or a membrane-bound nitrate reductase: does Nap confer a selective advantage during nitrate-limited growth?
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 151 citations in OpenAlex.
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- A Cytochrome c-Containing Periplasmic Nitrate Reductase in the Acetogen Sporomusa ovata.Environmental microbiology · 2026Article
- Metabolic labour division trade-offs in denitrifying microbiomes.The ISME journal · 2025Review
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- Environmentally dependent interactions shape patterns in gene content across natural microbiomes.Nature microbiology · 2024Article
- [Effect of intestinal nitrate on growth ofNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2024Article
- Transcriptional and Metabolic Response of a Strain ofBiotech (Basel (Switzerland)) · 2024Article
- Aerobic denitrification as an N2O source from microbial communities.The ISME journal · 2024Article
- The Genome ofMicroorganisms · 2023Article
- Subsurface biogeochemical cycling of nitrogen in the actively serpentinizing Samail Ophiolite, Oman.Frontiers in microbiology · 2023Article
- The Metabolic Adaptation in Response to Nitrate Is Critical for Actinobacillus pleuropneumoniae Growth and Pathogenicity under the Regulation of NarQ/P.Infection and immunity · 2022Article
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- Metaproteogenomic Profiling of Chemosynthetic Microbial Biofilms Reveals Metabolic Flexibility During Colonization of a Shallow-Water Gas Vent.Frontiers in microbiology · 2021Article
- Active sulfur cycling in the terrestrial deep subsurface.The ISME journal · 2020Article
- Molybdenum Enzymes and How They Support Virulence in Pathogenic Bacteria.Frontiers in microbiology · 2020Review
- Clustering as a Means To Control Nitrate Respiration Efficiency and Toxicity in Escherichia coli.mBio · 2019Article
- Article
- Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota.Frontiers in microbiology · 2018Article
- Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health.Free radical biology & medicine · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The physiological role of the periplasmic nitrate reductase, Nap, one of the three nitrate reductases synthesized by Escherichia coli K-12, has been investigated. A series of double mutants that express only one nitrate reductase were grown anaerobically in batch cultures with glycerol as the non-fermentable carbon source and nitrate as the terminal electron acceptor. Only the strain expressing nitrate reductase A grew rapidly under these conditions. Introduction of a narL mutation severely decreased the growth rate of the nitrate reductase A strain, but enhanced the growth of the Nap(+) strain. The ability to use nitrate as a terminal electron acceptor for anaerobic growth is therefore regulated primarily by the NarL protein at the level of transcription. Furthermore, the strain expressing nitrate reductase A had a substantial selective advantage in competition with the strain expressing only Nap during nitrate-sufficient continuous culture. However, the strain expressing Nap was preferentially selected during nitrate-limited continuous growth. The saturation constants for nitrate for the two strains (which numerically are equal to the nitrate concentrations at half of the maximum specific growth rate and therefore reflect the relative affinities for nitrate) were estimated using the integrated Monod equation to be 15 and 50 microM for Nap and nitrate reductase A respectively. This difference is sufficient to explain the selective advantage of the Nap(+) strain during nitrate-limited growth. It is concluded that one physiological role of the periplasmic nitrate reductase of enteric bacteria is to enable bacteria to scavenge nitrate in nitrate-limited environments.
Indexed as
Identifiers
10548536PMC1220616W2018755204What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.