ArticleThe Biochemical journal1999
Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 95 citations in OpenAlex.
- Expression Analysis of Nitrogen Metabolism Genes in Lelliottia amnigena PTJIIT1005, Comparison with Escherichia coli K12 and Validation of Nitrogen Metabolism Genes.Biochemical genetics · 2024Article
- Vibrio natriegens as a superior host for the production of c-type cytochromes and difficult-to-express redox proteins.Scientific reports · 2024Article
- Temperature, pH, and oxygen availability contributed to the functional differentiation of ancient Nitrososphaeria.The ISME journal · 2024Article
- History of Maturation of Prokaryotic Molybdoenzymes-A Personal View.Molecules (Basel, Switzerland) · 2023Review
- Subsurface biogeochemical cycling of nitrogen in the actively serpentinizing Samail Ophiolite, Oman.Frontiers in microbiology · 2023Article
- NapB Restores cytochrome c biosynthesis in bacterial dsbD-deficient mutants.Communications biology · 2022Article
- Biosynthesis of inorganic nanomaterials using microbial cells and bacteriophages.Nature reviews. Chemistry · 2020Review
- Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and COMicrobial cell factories · 2019Article
- Genome-Based Metabolic Reconstruction of a Novel Uncultivated Freshwater Magnetotactic coccus "Frontiers in microbiology · 2019Article
- Functional Redundancy in Perchlorate and Nitrate Electron Transport Chains and Rewiring Respiratory Pathways to Alter Terminal Electron Acceptor Preference.Frontiers in microbiology · 2018Article
- Nitrogen Metabolism Genes from Temperate Marine Sediments.Marine biotechnology (New York, N.Y.) · 2017Article
- Nitrate, nitrite and nitric oxide reductases: from the last universal common ancestor to modern bacterial pathogens.Current opinion in microbiology · 2016Review
- Genome sequence of Shimia str. SK013, a representative of the Roseobacter group isolated from marine sediment.Standards in genomic sciences · 2016Article
- The Periplasmic Nitrate Reductase NapABC Supports Luminal Growth of Salmonella enterica Serovar Typhimurium during Colitis.Infection and immunity · 2015Article
- Biosynthesis and Insertion of the Molybdenum Cofactor.EcoSal Plus · 2015Review
- It takes two to tango: two TatA paralogues and two redox enzyme-specific chaperones are involved in the localization of twin-arginine translocase substrates in Campylobacter jejuni.Microbiology (Reading, England) · 2014Article
- The mononuclear molybdenum enzymes.Chemical reviews · 2014Review
- Nitrate and periplasmic nitrate reductases.Chemical Society reviews · 2014Review
- Overlapping transport and chaperone-binding functions within a bacterial twin-arginine signal peptide.Molecular microbiology · 2012Article
- Four PCR primers necessary for the detection of periplasmic nitrate reductase genes in all groups of Proteobacteria and in environmental DNA.Biochemical Society transactions · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The seven nap genes at minute 47 on the Escherichia coli K-12 chromosome encode a functional nitrate reductase located in the periplasm. The molybdoprotein, NapA, is known to be essential for nitrate reduction. We now demonstrate that the two c-type cytochromes, the periplasmic NapB and the membrane-associated NapC, as well as a fourth polypeptide, NapD, are also essential for nitrate reduction in the periplasm by physiological substrates such as glycerol, formate and glucose. None of the three iron-sulphur proteins, NapF, NapG or NapH, are essential, irrespective of whether the bacteria are grown anaerobically in the presence of nitrate or fumarate as a terminal electron acceptor, or by glucose fermentation. Mutation of napD resulted in the total loss of Methyl Viologen-dependent nitrate reductase activity of the molybdoprotein, NapA, consistent with an earlier suggestion by others that NapD might be required for post-translational modification of NapA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.