ArticleThe Biochemical journal1999
Role of cysteine in the dietary control of the expression of 3-phosphoglycerate dehydrogenase in rat liver.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Dietary sulfur amino acid restriction improves glucose homeostasis through hepatic de novo serine synthesis.Molecular metabolism · 2026Article
- Dietary sulfur amino acid restriction improves glucose homeostasis through hepaticbioRxiv : the preprint server for biology · 2025Article
- Role of Impaired Glycolysis in Perturbations of Amino Acid Metabolism in Diabetes Mellitus.International journal of molecular sciences · 2023Review
- Cysteine restriction-specific effects of sulfur amino acid restriction on lipid metabolism.Aging cell · 2022Article
- Serine Metabolism in Health and Disease and as a Conditionally Essential Amino Acid.Nutrients · 2022Review
- Hepatocyte-SpecificNutrients · 2021Article
- Sulfur as a signaling nutrient through hydrogen sulfide.Annual review of nutrition · 2014Review
- Resurgence of serine: an often neglected but indispensable amino Acid.The Journal of biological chemistry · 2012Review
- Metabolic and genomic response to dietary isocaloric protein restriction in the rat.The Journal of biological chemistry · 2011Article
- The logic of the hepatic methionine metabolic cycle.Biochimica et biophysica acta · 2010Review
- L-serine in disease and development.The Biochemical journal · 2003Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shifting rats to a protein-free, carbohydrate-rich diet, although not starvation, resulted in the appearance of mRNA for, and activity of, 3-phosphoglycerate dehydrogenase (3-PGDH) in liver as well as in a marked decrease in plasma cystine concentration. Refeeding with protein caused a 50% decrease in the mRNA in 8 h and its complete disappearance within 24 h, followed by a slower disappearance of the enzymic activity. Intraperitoneal administration of cysteine or methionine to protein-starved rats decreased the mRNA by 50-60% after 8 h. However, the repeated administration of cysteine failed to cause the complete disappearance of this mRNA in 24 h. In hepatocytes in primary culture, cysteine plus methionine and glucagon had, independently, an approx. 4-fold inhibitory effect on the abundance of the 3-PGDH mRNA and caused its almost complete disappearance when tested together. Insulin had an approx. 2-fold stimulatory effect, which was antagonized by cysteine plus methionine but was still apparent in the presence of glucagon. Nuclear run-on experiments and analysis of the stability of the mRNA with 5,6-dichlorobenzimidazole riboside, an inhibitor of RNA polymerase II, suggested that the effect of cysteine plus methionine was due to destabilization of the mRNA, whereas the effect of glucagon was exerted on transcription. Cysteine, but not methionine, inhibited the accumulation of 3-PGDH mRNA in FTO2B hepatoma cells. In conclusion, the dietary control of the expression of the 3-PGDH gene in liver seems to involve the negative effects of cysteine and glucagon and the positive effect of insulin.
Indexed as
Identifiers
10548528PMC1220608W2062760324What OpenQuestion holds
Registered trials
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