ArticleThe Journal of cell biology1999
Reconstitution of membrane transport powered by a novel dimeric kinesin motor of the Unc104/KIF1A family purified from Dictyostelium.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Antisense oligonucleotides to KIF1A polymorphisms expand targets and rescue patient-derived neurons in vitro.Nature communications · 2026Article
- On and off controls within dynein-dynactin on native cargoes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A CRISPR-based method for testing the essentiality of a gene.Scientific reports · 2020Article
- These motors were made for walking.Protein science : a publication of the Protein Society · 2020Review
- Review
- Intracellular Cargo Transport by Kinesin-3 Motors.Biochemistry. Biokhimiia · 2017Review
- Dynein Clusters into Lipid Microdomains on Phagosomes to Drive Rapid Transport toward Lysosomes.Cell · 2016Article
- Tug-of-war between dissimilar teams of microtubule motors regulates transport and fission of endosomes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Traffic control: regulation of kinesin motors.Nature reviews. Molecular cell biology · 2009Review
- Microtubule-nucleus interactions in Dictyostelium discoideum mediated by central motor kinesins.Eukaryotic cell · 2009Article
- The Aspergillus nidulans kinesin-3 UncA motor moves vesicles along a subpopulation of microtubules.Molecular biology of the cell · 2009Article
- No conventional function for the conventional kinesin?Traffic (Copenhagen, Denmark) · 2008Article
- Disruption of four kinesin genes in dictyostelium.BMC cell biology · 2008Article
- Myosin-V, Kinesin-1, and Kinesin-3 cooperate in hyphal growth of the fungus Ustilago maydis.Molecular biology of the cell · 2005Article
- Role of Unc104/KIF1-related motor proteins in mitochondrial transport in Neurospora crassa.Molecular biology of the cell · 2005Article
- The lipid binding pleckstrin homology domain in UNC-104 kinesin is necessary for synaptic vesicle transport in Caenorhabditis elegans.Molecular biology of the cell · 2004Article
- Identification and phylogenetic analysis of Dictyostelium discoideum kinesin proteins.BMC genomics · 2003Article
- Article
- Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum.Molecular biology of the cell · 2002Article
- Kinesin motors and microtubule-based organelle transport in Dictyostelium discoideum.Journal of muscle research and cell motility · 2002Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Motor-powered movement along microtubule tracks is important for membrane organization and trafficking. However, the molecular basis for membrane transport is poorly understood, in part because of the difficulty in reconstituting this process from purified components. Using video microscopic observation of organelle transport in vitro as an assay, we have purified two polypeptides (245 and 170 kD) from Dictyostelium extracts that independently reconstitute plus-end-directed membrane movement at in vivo velocities. Both polypeptides were found to be kinesin motors, and the 245-kD protein (DdUnc104) is a close relative of Caenorhabditis elegans Unc104 and mouse KIF1A, neuron-specific motors that deliver synaptic vesicle precursors to nerve terminals. A knockout of the DdUnc104 gene produces a pronounced defect in organelle transport in vivo and in the reconstituted assay. Interestingly, DdUnc104 functions as a dimeric motor, in contrast to other members of this kinesin subfamily, which are monomeric.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.