ArticleThe Journal of cell biology1999
HCP-1, a protein involved in chromosome segregation, is localized to the centromere of mitotic chromosomes in Caenorhabditis elegans.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 81 citations in OpenAlex.
- Kinetochore components are perinuclear and form condensates inbioRxiv : the preprint server for biology · 2025Article
- Kinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes.Nature communications · 2023Article
- Functional midbody assembly in the absence of a central spindle.The Journal of cell biology · 2022Article
- An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes.G3 (Bethesda, Md.) · 2021Article
- Oligopaint DNA FISH reveals telomere-based meiotic pairing dynamics in the silkworm, Bombyx mori.PLoS genetics · 2021Article
- The Centromere Histone Is Conserved and Associated with Tandem Repeats Sharing a Conserved 19-bp Box in the Holocentromere of Meloidogyne Nematodes.Molecular biology and evolution · 2021Article
- Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development.PLoS genetics · 2019Article
- Mitotic Cell Division inGenetics · 2019Review
- NudE regulates dynein at kinetochores but is dispensable for other dynein functions in theJournal of cell science · 2018Article
- A novel chromosome segregation mechanism during female meiosis.Molecular biology of the cell · 2016Article
- Holocentromeres in Rhynchospora are associated with genome-wide centromere-specific repeat arrays interspersed among euchromatin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans.The Journal of cell biology · 2015Article
- Alternative meiotic chromatid segregation in the holocentric plant Luzula elegans.Nature communications · 2014Article
- Caenorhabditis elegans cyclin B3 is required for multiple mitotic processes including alleviation of a spindle checkpoint-dependent block in anaphase chromosome segregation.PLoS genetics · 2010Article
- Spindle assembly checkpoint genes reveal distinct as well as overlapping expression that implicates MDF-2/Mad2 in postembryonic seam cell proliferation in Caenorhabditis elegans.BMC cell biology · 2010Article
- Suspended animation extends survival limits of Caenorhabditis elegans and Saccharomyces cerevisiae at low temperature.Molecular biology of the cell · 2010Article
- A monoclonal antibody toolkit for C. elegans.PloS one · 2010Article
- NPP-16/Nup50 function and CDK-1 inactivation are associated with anoxia-induced prophase arrest in Caenorhabditis elegans.Molecular biology of the cell · 2010Article
- Hydrogen sulfide increases hypoxia-inducible factor-1 activity independently of von Hippel-Lindau tumor suppressor-1 in C. elegans.Molecular biology of the cell · 2010Article
- Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome.Molecular biology of the cell · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To learn more about holocentric chromosome structure and function, we generated a monoclonal antibody (mAb), 6C4, that recognizes the poleward face of mitotic chromosomes in Caenorhabditis elegans. Early in mitosis, mAb 6C4 stains dots throughout the nucleoplasm. Later in prophase, mAb 6C4 stains structures on opposing faces of chromosomes which orient towards the centrosomes at metaphase. Colocalization with an antibody against a centromeric histone H3-like protein and the MPM-2 antibody, which identifies a kinetochore-associated phosphoepitope present in a variety of organisms, shows that the mAb 6C4 staining is present adjacent to the centromere. Expression screening using mAb 6C4 identified a protein in C. elegans that we named HCP-1 (for holocentric protein 1). We also identified a second protein from the C. elegans genome sequence database, HCP-2, that is 54% similar to HCP-1. When expression of HCP-1 is reduced by RNA interference (RNAi), staining with mAb 6C4 is eliminated, indicating that hcp-1 encodes the major mAb 6C4 antigen. RNAi with hcp-1 and hcp-2 together results in aberrant anaphases and embryonic arrest at approximately 100 cells with different amounts of DNA in individual nuclei. These results suggest that HCP-1 is a centromere-associated protein that is involved in the fidelity of chromosome segregation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.