ArticleGenetics1999
RNA interference can target pre-mRNA: consequences for gene expression in a Caenorhabditis elegans operon.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 106 citations in OpenAlex.
- Transposon Regulation in thebioRxiv : the preprint server for biology · 2025Article
- Peri-centrosomal localization of small interfering RNAs in C. elegans.Science China. Life sciences · 2025Article
- The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates.Nature cell biology · 2022Article
- Labelling of Active Transcription Sites with Argonaute NRDE-3-Image Active Transcription SitesBio-protocol · 2022Article
- Article
- A Conserved NRDE-2/MTR-4 Complex Mediates Nuclear RNAi inGenetics · 2020Article
- A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans.Cell reports · 2020Article
- A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.Molecular cell · 2020Article
- Ago HITS-CLIP expands microRNA-mRNA interactions in nucleus and cytoplasm of gastric cancer cells.BMC cancer · 2019Article
- Article
- The Caenorhabditis elegans Ortholog of TDP-43 Regulates the Chromatin Localization of the Heterochromatin Protein 1 Homolog HPL-2.Molecular and cellular biology · 2018Article
- The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofPeerJ · 2017Article
- A Forward Genetic Screen for Suppressors of Somatic P Granules in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2015Article
- The MicroRNA Biology of the Mammalian Nucleus.Molecular therapy. Nucleic acids · 2014Article
- Nematode endogenous small RNA pathways.Worm · 2014Review
- The nuclear argonaute NRDE-3 contributes to transitive RNAi in Caenorhabditis elegans.Genetics · 2013Article
- Precise RNAi-mediated silencing of metabolically active proteins in the defence secretions of juvenile leaf beetles.Proceedings. Biological sciences · 2012Article
- A pre-mRNA-associating factor links endogenous siRNAs to chromatin regulation.PLoS genetics · 2011Article
- RNAi experiments in D. melanogaster: solutions to the overlooked problem of off-targets shared by independent dsRNAs.PloS one · 2010Article
- Alternative trans-splicing of Caenorhabditis elegans sma-9/schnurri generates a short transcript that provides tissue-specific function in BMP signaling.BMC molecular biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In nematodes, flies, trypanosomes, and planarians, introduction of double-stranded RNA results in sequence-specific inactivation of gene function, a process termed RNA interference (RNAi). We demonstrate that RNAi against the Caenorhabditis elegans gene lir-1, which is part of the lir-1/lin-26 operon, induced phenotypes very different from a newly isolated lir-1 null mutation. Specifically, lir-1(RNAi) induced embryonic lethality reminiscent of moderately strong lin-26 alleles, whereas the lir-1 null mutant was viable. We show that the lir-1(RNAi) phenotypes resulted from a severe loss of lin-26 gene expression. In addition, we found that RNAi directed against lir-1 or lin-26 introns induced similar phenotypes, so we conclude that lir-1(RNAi) targets the lir-1/lin-26 pre-mRNA. This provides direct evidence that RNA interference can prevent gene expression by targeting nuclear transcripts. Our results highlight that caution may be necessary when interpreting RNA interference without the benefit of mutant alleles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.