ArticleBiophysical journal1999
Real-time imaging of the dynamics of secretory granules in growth cones.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Axonal Transport of Lysosomes Is Unaffected in Glucocerebrosidase-Inhibited iPSC-Derived Forebrain Neurons.eNeuro · 2023Article
- Stochastic Subcellular Organization of Dense-Core Vesicles Revealed by Point Pattern Analysis.Biophysical journal · 2016Article
- Dendritic and axonal mechanisms of Ca2+ elevation impair BDNF transport in Aβ oligomer-treated hippocampal neurons.Molecular biology of the cell · 2015Article
- Amyloid-β oligomers induce tau-independent disruption of BDNF axonal transport via calcineurin activation in cultured hippocampal neurons.Molecular biology of the cell · 2013Article
- Hindered submicron mobility and long-term storage of presynaptic dense-core granules revealed by single-particle tracking.Developmental neurobiology · 2012Article
- The plasminogen activation system and the regulation of catecholaminergic function.Journal of biomedicine & biotechnology · 2012Review
- Expression of the dominant-negative tail of myosin Va enhances exocytosis of large dense core vesicles in neurons.Cellular and molecular neurobiology · 2009Article
- Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells.Histochemistry and cell biology · 2008Article
- Efficient copackaging and cotransport yields postsynaptic colocalization of neuromodulators associated with synaptic plasticity.Developmental neurobiology · 2008Article
- Rab8 regulates the actin-based movement of melanosomes.Molecular biology of the cell · 2005Article
- Mechanisms of transport and exocytosis of dense-core granules containing tissue plasminogen activator in developing hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Uncoated endocytic vesicles require the unconventional myosin, Myo6, for rapid transport through actin barriers.Molecular biology of the cell · 2004Article
- Unexpected mobility variation among individual secretory vesicles produces an apparent refractory neuropeptide pool.Biophysical journal · 2003Article
- Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 cells.The Journal of physiology · 2002Article
- Nerve growth factor-induced differentiation changes the cellular organization of regulated Peptide release by PC12 cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Actin filaments and myosin I alpha cooperate with microtubules for the movement of lysosomes.Molecular biology of the cell · 2001Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretory granules containing a hybrid protein consisting of the regulated secretory protein tissue plasminogen activator and an enhanced form of green fluorescent protein were tracked at high spatial resolution in growth cones of differentiated PC12 cells. Tracking shows that granules, unlike synaptic vesicles, generally are mobile in growth cones. Quantitative analysis of trajectories generated by granules revealed two dominant modes of motion: diffusive and directed. Diffusive motion was observed primarily in central and peripheral parts of growth cones, where most granules diffused two to four orders of magnitude more slowly than comparably sized spheres in dilute solution. Directed motion was observed primarily in proximal parts of growth cones, where a subset of granules underwent rapid, directed motion at average speeds comparable to those observed for granules in neurites. This high-resolution view of the dynamics of secretory granules in growth cones provides insight into granule organization and release at nerve terminals. In particular, the mobility of granules suggests that granules, unlike synaptic vesicles, are not tethered stably to cytoskeletal structures in nerve terminals. Moreover, the slow diffusive nature of this mobility suggests that secretory responses involving centrally distributed granules in growth cones will occur slowly, on a time scale of minutes or longer.
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