ArticleBiophysical journal1999
Single-molecule anisotropy imaging.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Microfluidic measurement of the size and shape of lipid-anchored proteins.Biophysical journal · 2024Article
- Mapping fast DNA polymerase exchange during replication.Nature communications · 2024Article
- Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue.Disease models & mechanisms · 2022Article
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- Ratiometric widefield imaging with spectrally balanced detection.Biomedical optics express · 2019Article
- mmSTORM: Multimodal localization based super-resolution microscopy.Scientific reports · 2019Article
- Monomeric TCRs drive T cell antigen recognition.Nature immunology · 2018Article
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- The transition from single molecule to ensemble revealed by fluorescence polarization.Scientific reports · 2015Article
- A starting point for fluorescence-based single-molecule measurements in biomolecular research.Molecules (Basel, Switzerland) · 2014Review
- Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles.Frontiers in physiology · 2014Review
- Four-dimensional spatial nanometry of single particles in living cells using polarized quantum rods.Biophysical journal · 2013Article
- Rotational diffusion of the α(2a) adrenergic receptor revealed by FlAsH labeling in living cells.Biophysical journal · 2011Article
- Codon-dependent tRNA fluctuations monitored with fluorescence polarization.Biophysical journal · 2010Article
- Characterization of horizontal lipid bilayers as a model system to study lipid phase separation.Biophysical journal · 2010Article
- Fluorescence polarization/anisotropy in diagnostics and imaging.Chemical reviews · 2010Review
- Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate.Biophysical journal · 2009Article
- Diffusion in supported lipid bilayers: influence of substrate and preparation technique on the internal dynamics.The European physical journal. E, Soft matter · 2009Article
- Local viscosity of supercooled glycerol near Tg probed by rotational diffusion of ensembles and single dye molecules.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
5 authors.
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Abstract
A novel method, single-molecule anisotropy imaging, has been employed to simultaneously study lateral and rotational diffusion of fluorescence-labeled lipids on supported phospholipid membranes. In a fluid membrane composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, in which the rotational diffusion time is on the order of the excited-state lifetime of the fluorophore rhodamine, a rotational diffusion constant, D(rot) = 7 x 10(7) rad(2)/s, was determined. The lateral diffusion constant, measured by direct analysis of single-molecule trajectories, was D(lat) = 3.5 x 10(-8) cm(2)/s. As predicted from the free-volume model for diffusion, the results exhibit a significantly enhanced mobility on the nanosecond time scale. For membranes of DPPC lipids in the L(beta) gel phase, the slow rotational mobility permitted the direct observation of the rotation of individual molecules characterized by D(rot) = 1.2 rad(2)/s. The latter data were evaluated by a mean square angular displacement analysis. The technique developed here should prove itself profitable for imaging of conformational motions of individual proteins on the time scale of milliseconds to seconds.
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