ArticleThe Journal of physiology1999
Opposite effects of pH on open-state probability and single channel conductance of kir4.1 channels.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Cell Settling, Migration, and Stochastic Cancer Gene Expression Suggest Potassium Membrane Flux May Initiate pH Reversal.Biomolecules · 2025Article
- CaFrontiers in cellular neuroscience · 2023Article
- Novel KCNJ10 Gene Variations Compromise Function of Inwardly Rectifying Potassium Channel 4.1.The Journal of biological chemistry · 2016Article
- Ion dynamics during seizures.Frontiers in cellular neuroscience · 2015Review
- S-Glutathionylation underscores the modulation of the heteromeric Kir4.1-Kir5.1 channel in oxidative stress.The Journal of physiology · 2012Article
- Renal phenotype in mice lacking the Kir5.1 (Kcnj16) K+ channel subunit contrasts with that observed in SeSAME/EAST syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The salt-wasting phenotype of EAST syndrome, a disease with multifaceted symptoms linked to the KCNJ10 K+ channel.Pflugers Archiv : European journal of physiology · 2011Review
- Molecular basis of decreased Kir4.1 function in SeSAME/EAST syndrome.Journal of the American Society of Nephrology : JASN · 2010Article
- KCNJ10 gene mutations causing EAST syndrome (epilepsy, ataxia, sensorineural deafness, and tubulopathy) disrupt channel function.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- High CO2 chemosensitivity versus wide sensing spectrum: a paradoxical problem and its solutions in cultured brainstem neurons.The Journal of physiology · 2007Article
- Kir6.2 channel gating by intracellular protons: subunit stoichiometry for ligand binding and channel gating.The Journal of membrane biology · 2006Article
- Determinant role of membrane helices in K ATP channel gating.The Journal of membrane biology · 2005Article
- Molecular diversity and regulation of renal potassium channels.Physiological reviews · 2005Review
- Expression and coexpression of CO2-sensitive Kir channels in brainstem neurons of rats.The Journal of membrane biology · 2004Article
- Regulation of inwardly rectifying K+ channels in retinal pigment epithelial cells by intracellular pH.The Journal of physiology · 2003Article
- Structural and functional determinants of conserved lipid interaction domains of inward rectifying Kir6.2 channels.The Journal of general physiology · 2002Article
- Specific localization of an inwardly rectifying K(+) channel, Kir4.1, at the apical membrane of rat gastric parietal cells; its possible involvement in K(+) recycling for the H(+)-K(+)-pump.The Journal of physiology · 2002Article
- An inward rectifier K(+) channel at the basolateral membrane of the mouse distal convoluted tubule: similarities with Kir4-Kir5.1 heteromeric channels.The Journal of physiology · 2002Article
- Differential pH sensitivity of Kir4.1 and Kir4.2 potassium channels and their modulation by heteropolymerisation with Kir5.1.The Journal of physiology · 2001Article
- A single residue contributes to the difference between Kir4.1 and Kir1.1 channels in pH sensitivity, rectification and single channel conductance.The Journal of physiology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. A decrease in intracellular pH (pHi) inhibits whole-cell Kir4.1 currents. To understand channel biophysical properties underlying this inhibition, single channel Kir4.1 currents were studied in inside-out patches using symmetric concentrations of K+ applied to each side of the plasma membrane. Under such conditions, inward rectifying currents were observed in about 2 of 3 patches. At pH 7.4, these currents showed a single channel conductance of 22 pS with a channel open-state probability (Popen) of approximately 0.9. 2. The effects of intracellular protons on macroscopic Kir4.1 currents were examined in giant inside-out patches at various pH levels of internal solutions. Current amplitude increased with a modest acidification (pH 7.0 and 6.6), and decreased with further reductions in pHi. The Kir4.1 currents were completely suppressed at pH 5.4. These effects were fast and reversible. 3. Low pHi inhibited Popen and enhanced single channel conductance in a concentration-dependent manner with pK (midpoint pH value for channel inhibition) of 6.0 and 6.8, respectively. At pH 5.8, Popen was inhibited by 70 % and single channel conductance increased by 35 %. Washout brought both Popen and single channel conductance rapidly back to baseline levels. 4. Theoretical currents were calculated using percentage changes in Popen and single channel conductance at each pH level tested. The trajectory of these currents is very close to that of experimental currents recorded from giant patches. Thus, opposite effects of intracellular protons on Popen and single channel conductance are demonstrated, which are likely to result in changes of macroscopic Kir4.1 currents with low pH.
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