ArticleThe Journal of physiology1999
Mode of Ca2+ action on ciliary beat frequency in single ovine airway epithelial cells.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 83 citations in OpenAlex.
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- Selective amino acid formulation enhances anion secretion and restores function in cystic fibrosis mutations.Frontiers in pharmacology · 2025Article
- Increased Muc5AC and Decreased Ciliated Cells in Severe Asthma Partially Restored by Inhibition of IL-4Rα Receptor.American journal of respiratory and critical care medicine · 2024Article
- Sex differences in airway disease: estrogen and airway surface liquid dynamics.Biology of sex differences · 2024Review
- A fixed 20:1 combination of cafedrine/theodrenaline increases cytosolic CaScientific reports · 2023Article
- E-cigarette aerosols of propylene glycol impair BK channel activity and parameters of mucociliary function.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Mucociliary Wnt signaling promotes cilia biogenesis and beating.Nature communications · 2023Article
- The CFTR Amplifier Nesolicaftor Rescues TGF-β1 Inhibition of Modulator-Corrected F508del CFTR Function.International journal of molecular sciences · 2022Article
- Vegetable glycerin e-cigarette aerosols cause airway inflammation and ion channel dysfunction.Frontiers in pharmacology · 2022Article
- Losartan reduces cigarette smoke-induced airway inflammation and mucus hypersecretion.ERJ open research · 2021Article
- A low [CaPflugers Archiv : European journal of physiology · 2017Article
- Marked increases in mucociliary clearance produced by synergistic secretory agonists or inhibition of the epithelial sodium channel.Scientific reports · 2016Article
- Roflumilast partially reverses smoke-induced mucociliary dysfunction.Respiratory research · 2015Article
- Airway Surface Dehydration by Transforming Growth Factor β (TGF-β) in Cystic Fibrosis Is Due to Decreased Function of a Voltage-dependent Potassium Channel and Can Be Rescued by the Drug Pirfenidone.The Journal of biological chemistry · 2015Article
- Rhythmicity, recurrence, and recovery of flagellar beating.Physical review letters · 2014Article
- Soluble adenylyl cyclase in health and disease.Biochimica et biophysica acta · 2014Review
- IFN-γ-mediated reduction of large-conductance, Ca2+-activated, voltage-dependent K+ (BK) channel activity in airway epithelial cells leads to mucociliary dysfunction.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Methods to measure and analyze ciliary beat activity: Ca2+ influx-mediated cilia mechanosensitivity.Pflugers Archiv : European journal of physiology · 2012Article
- Computational modelling elucidates the mechanism of ciliary regulation in health and disease.BMC systems biology · 2011Article
- Functional apical large conductance, Ca2+-activated, and voltage-dependent K+ channels are required for maintenance of airway surface liquid volume.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. We analysed the kinetics of coupling between cytoplasmic calcium ([Ca2+]i) and ciliary beat frequency (CBF) using simultaneous single cilium recording and single cell [Ca2+]i measurements from cultured ovine tracheal epithelial cells. 2. CBF and [Ca2+]i (indicated by fura-2) were measured at rest and in response to activation of the G-protein coupled M3 muscarinic receptor by 10 microM acetylcholine (ACh). 3. Fourier transform analysis of 3 s data segments of light intensity from phase-contrast microscopy showed no significant delay between changes in [Ca2+]i and CBF during a 2 min exposure to ACh and subsequent washout. 4. CBF time resolution was improved by computing instantaneous beat frequency. This revealed that CBF lagged the rapid increase in [Ca2+]i in response to ACh with a delay of less than 1 beat cycle (143 ms at 7 Hz). When CBF was estimated by an improved Fourier method, this delay was observed to be 70 +/- 30 ms (mean +/- s.e.m.; n = 20 cilia). During the slower return to baseline, a lag of 8 +/- 3.2 s was observed, indicative of hysteresis. 5. While calmodulin inhibitors (calmidazolium and W-7; each n = 5) decreased baseline CBF by an average of 1.1 +/- 0.1 Hz, they did not alter the kinetic relationship between [Ca2+]i and CBF. Similarly, phosphatase inhibitors (okadaic acid and cyclosporin A; each n = 5), changed neither baseline CBF nor the kinetic coupling between [Ca2+]i and CBF. 6. These data suggest that the timing of Ca2+ action on CBF in ovine airway epithelial cells, is unlikely to be determined by phosphorylation reactions involving calmodulin or kinase/phosphatase reactions. 7. A simple model for Ca2+ stimulation of CBF is presented. Fits of the model to the data suggest four or more Ca2+ ions bind cooperatively to speed up CBF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.