ArticleThe Journal of physiology1999
Purinergic regulation of cation conductances and intracellular Ca2+ in cultured rat retinal pigment epithelial cells.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Mechanisms of Ion Transport Across the Mouse Retinal Pigment Epithelium Measured In Vitro.Investigative ophthalmology & visual science · 2020Article
- Regulatory mechanism for the transmembrane receptor that mediates bidirectional vitamin A transport.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications.Stem cells translational medicine · 2016Article
- GABAB receptors are expressed in human aortic smooth muscle cells and regulate the intracellular Ca(2+) concentration.Heart and vessels · 2015Article
- GABAAα1 and GABAAρ1 subunits are expressed in cultured human RPE cells and GABAA receptor agents modify the intracellular calcium concentration.Molecular vision · 2015Article
- GABAB receptors expressed in human aortic endothelial cells mediate intracellular calcium concentration regulation and endothelial nitric oxide synthase translocation.BioMed research international · 2014Article
- Activation of P2X receptors induces apoptosis in human retinal pigment epithelium.Investigative ophthalmology & visual science · 2011Article
- Functional Expression of P2Y Receptors in WERI-Rb1 Retinoblastoma Cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2011Article
- Expression of Orai genes and I(CRAC) activation in the human retinal pigment epithelium.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2011Article
- P2Y1 receptors mediate an activation of neuronal calcium-dependent K+ channels.The Journal of physiology · 2010Article
- The presence of bestrophin-1 modulates the Ca2+ recruitment from Ca2+ stores in the ER.Pflugers Archiv : European journal of physiology · 2010Article
- Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium.Molecular vision · 2010Article
- Purinergic signalling in the subretinal space: a role in the communication between the retina and the RPE.Purinergic signalling · 2008Article
- Voltage-dependent Ca2+ channels, not ryanodine receptors, activate Ca2+-dependent BK potassium channels in human retinal pigment epithelial cells.Molecular vision · 2008Article
- Glutamate acts at NMDA receptors on fresh bovine and on cultured human retinal pigment epithelial cells to trigger release of ATP.The Journal of physiology · 2006Article
- P2Y(2) receptor agonist INS37217 enhances functional recovery after detachment caused by subretinal injection in normal and rds mice.Investigative ophthalmology & visual science · 2003Article
- Ca2+ signalling and membrane current activated by cADPr in starfish oocytes.Pflugers Archiv : European journal of physiology · 2003Article
- Coupling of a P2Z-like purinoceptor to a fatty acid-activated K(+) channel in toad gastric smooth muscle cells.The Journal of physiology · 2001Article
- Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space.The Journal of physiology · 2001Article
- Defective regulatory volume decrease in human cystic fibrosis tracheal cells because of altered regulation of intermediate conductance Ca2+-dependent potassium channels.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We used whole-cell patch clamp and fluorescent calcium imaging techniques to investigate the effects of adenosine 5'-triphosphate (ATP) on membrane currents and intracellular calcium concentration ([Ca2+]i)in rat retinal pigment epithelial (RPE) cells. In 62 % of RPE cells, application of 100 microM ATP elicited a fast inward current at negative membrane potentials. In 38 % of RPE cells recorded, a biphasic response to ATP was observed in which activation of the fast inward current was followed by activation of a delayed outward current. 2. The ATP-activated inward current was a non-selective cation (NSC) current that showed inward rectification, reversed at -1.5 +/- 1 mV and was permeable to monovalent cations. The NSC current was insensitive to the P2 purinoceptor antagonists, suramin or PPADS but was activated by the purinoceptor agonists UTP, ADP and 2MeSATP. 3. The outward current activated by ATP reversed at -68 +/- 3 mV (equilibrium potential for potassium (EK) = -84 mV) and was blocked by Ba2+ ions, consistent with the activation of a K+ conductance. The outward K+ conductance was also reduced by the maxi-KCa channel blocker iberiotoxin (IbTX; 10 nM), suggesting that ATP activated an outward Ca2+-activated K+ channel in rat RPE cells. The Ca2+-activated K+ current (IK(Ca)) was also activated by the purinoceptor agonists UTP, ADP and 2MeSATP. 4. In fluo-3 or fluo-4 loaded RPE cells, ATP and the pyrimidine agonist UTP elevated [Ca2+]i. The increase in Ca2+ was not dependent on extracellular Ca2+ influx, but was sensitive to the Ca2+-ATPase inhibitor thapsigargin, confirming the involvement of intracellular Ca2+ stores release. 5. These results suggest that rat RPE cells express both P2X purinoceptors that gate activation of a non-selective cation conductance and G protein-coupled P2Y purinoceptors that mediate Ca2+ release from intracellular stores and activation of a calcium-activated K+ current.
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