ArticleApplied and environmental microbiology1999
Inducible amplification of gene copy number and heterologous protein production in the yeast Kluyveromyces lactis.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 39 citations in OpenAlex.
- Evaluation of chromosomal insertion loci in the Pseudomonas putida KT2440 genome for predictable biosystems design.Metabolic engineering communications · 2020Article
- Thermal adaptability of Kluyveromyces marxianus in recombinant protein production.Microbial cell factories · 2013Article
- SOD1, a new Kluyveromyces lactis helper gene for heterologous protein secretion.Applied and environmental microbiology · 2008Article
- Induction by hypoxia of heterologous-protein production with the KlPDC1 promoter in yeasts.Applied and environmental microbiology · 2007Article
- Secretion of human serum albumin by Kluyveromyces lactis overexpressing KlPDI1 and KlERO1.Applied and environmental microbiology · 2005Article
- Improved production of heterologous proteins by a glucose repression-defective mutant of Kluyveromyces lactis.Applied and environmental microbiology · 2004Article
- Efficient homolactic fermentation by Kluyveromyces lactis strains defective in pyruvate utilization and transformed with the heterologous LDH gene.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid recombinase gene. We increased the production and secretion of two heterologous proteins, glucoamylase from the yeast Arxula adeninivorans and mammalian interleukin-1beta, following gene dosage amplification when the heterologous genes were carried by pKD1-based vectors. The choice of the promoters for expression of the integrated recombinase gene and of the episomal heterologous genes are critical for the mitotic stability of the host-vector system.
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Registered trials
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