ArticleJournal of bacteriology1999
Influence of S-adenosylmethionine pool size on spontaneous mutation, dam methylation, and cell growth of Escherichia coli.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- A versatile method for designing biosensors via regulatory domains of allosteric enzymes.Nature communications · 2026Article
- Temperature-dependent dynamics of DNA methylation and plasmid copy number in Yersinia pestis.BMC genomics · 2026Article
- Article
- Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level.Nucleic acids research · 2023Article
- Review
- Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.Nature communications · 2021Article
- Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.Nature communications · 2019Article
- A surprising range of modified-methionyl S-adenosylmethionine analogues support bacterial growth.Microbiology (Reading, England) · 2015Article
- Profiling substrates of protein arginine N-methyltransferase 3 with S-adenosyl-L-methionine analogues.ACS chemical biology · 2014Article
- The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein.Archives of biochemistry and biophysics · 2011Article
- Binding of MetJ repressor to specific and nonspecific DNA and effect of S-adenosylmethionine on these interactions.Biochemistry · 2010Article
- Growth deficiencies of Neisseria meningitidis pfs and luxS mutants are not due to inactivation of quorum sensing.Journal of bacteriology · 2009Article
- Transcriptional networks in S. cerevisiae linked to an accumulation of base excision repair intermediates.PloS one · 2007Article
- Amino acid residues of the Escherichia coli tRNA(m5U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity.Nucleic acids research · 2007Article
- Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.Journal of bacteriology · 2006Article
- A stochastic model of Escherichia coli AI-2 quorum signal circuit reveals alternative synthesis pathways.Molecular systems biology · 2006Article
- Health considerations regarding horizontal transfer of microbial transgenes present in genetically modified crops.Journal of biomedicine & biotechnology · 2005Article
- Evidence for a second class of S-adenosylmethionine riboswitches and other regulatory RNA motifs in alpha-proteobacteria.Genome biology · 2005Article
- Article
- Transcription termination control of the S box system: direct measurement of S-adenosylmethionine by the leader RNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli strains that are deficient in the Ada and Ogt DNA repair methyltransferases display an elevated spontaneous G:C-to-A:T transition mutation rate, and this increase has been attributed to mutagenic O(6)-alkylguanine lesions being formed via the alkylation of DNA by endogenous metabolites. Here we test the frequently cited hypothesis that S-adenosylmethionine (SAM) can act as a weak alkylating agent in vivo and that it contributes to endogenous DNA alkylation. By regulating the expression of the rat liver SAM synthetase and the bacteriophage T3 SAM hydrolase proteins in E. coli, a 100-fold range of SAM levels could be achieved. However, neither increasing nor decreasing SAM levels significantly affected spontaneous mutation rates, leading us to conclude that SAM is not a major contributor to the endogenous formation of O(6)-methylguanine lesions in E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.