ArticleGenes & development1999
Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
91 citing papers in PubMed, 205 citations in OpenAlex.
- Ordered and disordered regions of the Origin Recognition Complex direct differential in vivo binding at distinct motif sequences.Nucleic acids research · 2024Article
- The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man.Nucleic acids research · 2024Article
- Developmentally Programmed Switches in DNA Replication: Gene Amplification and Genome-Wide Endoreplication inMicroorganisms · 2023Article
- Review
- Nucleoporins facilitate ORC loading onto chromatin.Cell reports · 2022Article
- Anatomy and evolution of a DNA replication origin.Chromosoma · 2021Article
- Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6.Nature communications · 2020Article
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Origins of DNA replication.PLoS genetics · 2019Review
- DNA Replication Control DuringGenetics · 2017Review
- Initiation of Drosophila chorion gene amplification requires Claspin and mus101, whereas Claspin, but not mus101, plays a major role during elongation.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Article
- Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeastThe Journal of biological chemistry · 2017Article
- The Histone Variant H3.3 Is Enriched at Drosophila Amplicon Origins but Does Not Mark Them for Activation.G3 (Bethesda, Md.) · 2016Article
- Groucho restricts rhomboid expression and couples EGFR activation with R8 selection during Drosophila photoreceptor differentiation.Developmental biology · 2015Article
- DNA sequence templates adjacent nucleosome and ORC sites at gene amplification origins in Drosophila.Nucleic acids research · 2015Article
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.PloS one · 2015Article
- Prereplication-complex formation: a molecular double take?Nature structural & molecular biology · 2014Article
- Chromatin and DNA replication.Cold Spring Harbor perspectives in biology · 2013Review
- The histone acetyltransferases CBP and Chameau integrate developmental and DNA replication programs in Drosophila ovarian follicle cells.Development (Cambridge, England) · 2012Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In the yeast Saccharomyces cerevisiae, sequence-specific DNA binding by the origin recognition complex (ORC) is responsible for selecting origins of DNA replication. In metazoans, origin selection is poorly understood and it is unknown whether specific DNA binding by metazoan ORC controls replication. To address this problem, we used in vivo and in vitro approaches to demonstrate that Drosophila ORC (DmORC) binds to replication elements that direct repeated initiation of replication to amplify the Drosophila chorion gene loci in the follicle cells of egg chambers. Using immunolocalization, we observe that ACE3, a 440-bp chorion element that contains information sufficient to drive amplification, directs DmORC localization in follicle cells. Similarly, in vivo cross-linking and chromatin immunoprecipitation assays demonstrate association of DmORC with both ACE3 and two other amplification control elements, AER-d and ACE1. To demonstrate that the in vivo localization of DmORC is related to its DNA-binding properties, we find that purified DmORC binds to ACE3 and AER-d in vitro, and like its S. cerevisiae counterpart, this binding is dependent on ATP. Our findings suggest that sequence-specific DNA binding by ORC regulates initiation of metazoan DNA replication. Furthermore, adaptation of this experimental approach will allow for the identification of additional metazoan ORC DNA-binding sites and potentially origins of replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.