ArticleJournal of the American Society for Mass Spectrometry1999
Calcium-induced noncovalently linked tetramers of MRP8 and MRP14 detected by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 128 citations in OpenAlex.
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- Spectroscopic and computational investigations of Cobalt(II) binding to the innate immune protein human calprotectin.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2024Article
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- High-affinity peptides developed against calprotectin and their application as synthetic ligands in diagnostic assays.Nature communications · 2023Article
- Alarming and Calming: Opposing Roles of S100A8/S100A9 Dimers and Tetramers on Monocytes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- The role of S100A9 in the interaction between pancreatic ductal adenocarcinoma cells and stromal cells.Cancer immunology, immunotherapy : CII · 2022Article
- Molecular Basis of Ca(II)-Induced Tetramerization and Transition-Metal Sequestration in Human Calprotectin.Journal of the American Chemical Society · 2021Article
- Discerning the promising binding sites of S100/calgranulins and their therapeutic potential in atherosclerosis.Expert opinion on therapeutic patents · 2021Review
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- Neutrophil signaling during myocardial infarction wound repair.Cellular signalling · 2021Review
- Calcium Binding to the Innate Immune Protein Human Calprotectin Revealed by Integrated Mass Spectrometry.Journal of the American Chemical Society · 2020Article
- miRNAs Regulate Cytokine Secretion Induced by Phosphorylated S100A8/A9 in Neutrophils.International journal of molecular sciences · 2019Article
- Murine Calprotectin Coordinates Mn(II) at a Hexahistidine Site with Ca(II)-Dependent Affinity.Inorganic chemistry · 2019Article
- Oxidative cross-linking of calprotectin occurs in vivo, altering its structure and susceptibility to proteolysis.Redox biology · 2019Article
- Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Oxidative Post-translational Modifications Accelerate Proteolytic Degradation of Calprotectin.Journal of the American Chemical Society · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MRP8 and MRP14 are members of the S100 family of calcium-binding proteins which play an important role during calcium-induced activation of phagocytes. Both proteins form noncovalently associated complexes as a prerequisite for biological functions. The exact stoichiometric composition of these complexes, however, has not been completely clarified yet. In the present study we show for the first time by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) the calcium-induced formation of noncovalently associated (MRP8/MRP14)2 tetramers. Furthermore, we could determine posttranslational modifications of MRP8 and MRP14, the stoichiometric proportion of the two known MRP14 isoforms in the complexes as well as the number of calcium ions bound to the single MRP8 and MRP14 monomers and tetramers. MRP14 showed a higher affinity for calcium than MRP8. Upon complex formation the calcium binding increased to maximal saturation of the known EF hands in the complexed forms. Calcium-induced stabilization of the MRP8/MRP14 complexes was confirmed by DSC studies. Our results extend scope and application of UV-MALDI-MS by allowing identification of noncovalent protein complexes, the identification of minor alterations of subunits in such complexes as well as the determination of bound calcium ions.
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