ArticleProceedings of the National Academy of Sciences of the United States of America1999
Adeno-associated virus (AAV) site-specific integration: formation of AAV-AAVS1 junctions in an in vitro system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Improved Recombinant Adeno-Associated Viral Vector Production via Molecular Evolution of the Viral Rep Protein.International journal of molecular sciences · 2025Article
- Adeno-associated virus infection and its impact in human health: an overview.Virology journal · 2022Review
- Review
- Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus.Biochemistry · 2013Article
- My life with adeno-associated virus: a long time spent studying a short genome.DNA and cell biology · 2013Article
- Ribosomal DNA integrating rAAV-rDNA vectors allow for stable transgene expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- p53 inhibits adeno-associated viral vector integration.Human gene therapy · 2011Article
- Integration preferences of wildtype AAV-2 for consensus rep-binding sites at numerous loci in the human genome.PLoS pathogens · 2010Article
- Gene therapy for cystic fibrosis.Clinical reviews in allergy & immunology · 2008Review
- Adeno-associated virus site-specific integration is regulated by TRP-185.Journal of virology · 2007Article
- DNA-dependent PK inhibits adeno-associated virus DNA integration.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Evidence of chromosomal integration of AAV DNA in human testis tissue.Virus genes · 2004Article
- Identification and characterization of an adeno-associated virus integration site in CV-1 cells from the African green monkey.Journal of virology · 2003Article
- A p5 integration efficiency element mediates Rep-dependent integration into AAVS1 at chromosome 19.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Adeno-associated virus (AAV) site-specific recombination does not require a Rep-dependent origin of replication within the AAV terminal repeat.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Selective cleavage of AAVS1 substrates by the adeno-associated virus type 2 rep68 protein is dependent on topological and sequence constraints.Journal of virology · 2000Article
- DNA sequence motifs which direct adeno-associated virus site-specific integration in a model system.Journal of virology · 2000Article
- Roles of adeno-associated virus Rep protein and human chromosome 19 in site-specific recombination.Journal of virology · 2000Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
An in vitro system to study the mechanism of site-specific integration of adeno-associated virus (AAV) was developed. This system is based on two substrates, a linear or circular AAV donor and a circular acceptor containing the preintegration locus AAVS1. In the presence of HeLa extract and the His-Tag-purified Rep68 protein, specific covalent junctions between AAV and AAVS1 were formed and detected by PCR. The majority of the junctions were located within the Rep binding site of both the AAV and the AAVS1 substrates, underlining the involvement of the Rep protein. A limited amount of replication and the presence of nuclear factors promoted the efficiency of the reaction. The process was ATP-dependent, indicating that the helicase activity of Rep may be important in the formation of the junctions. According to current models of integration, the formation of the junctions would represent a first step in the process of AAV integration. This step could be crucial for the site specificity of the recombination event that leads to the integration of AAV into human chromosome 19 in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.