Evidence map›Paper›PMID 10536000›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America1999

Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: role of epsilon protein kinase C.

C H Chen, M O Gray, D Mochly-Rosen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 136 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Brain ethanol metabolism and mitochondria.Current topics in biochemical research · 2022
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Identification of εPKC targets during cardiac ischemic injury.Circulation journal : official journal of the Japanese Circulation Society · 2012
    Article
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Focus on: The cardiovascular system: what did we learn from the French (Paradox)?Alcohol research & health : the journal of the National Institute on Alcohol Abuse and Alcoholism · 2010
    Review
  18. Article
  19. Review
  20. Ethanol for cardiac ischemia: the role of protein kinase c.Therapeutic advances in cardiovascular disease · 2008
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

C H ChenDepartment of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305-5332, USA.
M O Gray
D Mochly-Rosen
Stanford University · USUniversity of California, San Francisco · US

Funding

MECHANISMS OF ETHANOL-INDUCED CARDIAC PROTECTIONR01AA011147 · NIAAA · STANFORD UNIVERSITY · PI DARIA MOCHLY-ROSEN · 1996 to 2026
$8.2M
Mechanisms of Ethanol-Induced Cardiac ProtectionR37AA011147 · NIAAA · STANFORD UNIVERSITY · PI MOCHLY-ROSEN, DARIA · 2009 to 2018
$5.3M
NIAAA NIH HHS AA11147NIAAA NIH HHS R01 AA011147NIAAA NIH HHS R37 AA011147
6 · The paper itself

Abstract

Recent epidemiological studies indicate beneficial effects of moderate ethanol consumption in ischemic heart disease. Most studies, however, focus on the effect of long-term consumption of ethanol. In this study, we determined whether brief exposure to ethanol immediately before ischemia also produces cardioprotection. In addition, because protein kinase C (PKC) has been shown to mediate protection of the heart from ischemia, we determined the role of specific PKC isozymes in ethanol-induced protection. We demonstrated that (i) brief exposure of isolated adult rat cardiac myocytes to 10-50 mM ethanol protected against damage induced by prolonged ischemia; (ii) an isozyme-selective epsilonPKC inhibitor developed in our laboratory inhibited the cardioprotective effect of acute ethanol exposure; (iii) protection of isolated intact adult rat heart also occurred after incubation with 10 mM ethanol 20 min before global ischemia; and (iv) ethanol-induced cardioprotection depended on PKC activation because it was blocked by chelerythrine and GF109203X, two PKC inhibitors. Consumption of 1-2 alcoholic beverages in humans leads to blood alcohol levels of approximately 10 mM. Therefore, our work demonstrates that exposure to physiologically attainable ethanol levels minutes before ischemia provides cardioprotection that is mediated by direct activation of epsilonPKC in the cardiac myocytes. The potential clinical implications of our findings are discussed.

Indexed as

Adhesins, BacterialAmino Acid SequenceAnimalsBlotting, WesternCarrier ProteinsDose-Response Relationship, DrugEnzyme InhibitorsEthanolIn Vitro TechniquesIsoenzymesMaleMolecular Sequence DataMyocardial IschemiaProtein Kinase CProtein Kinase C-epsilonRatsAdhesins, BacterialBabA protein, Helicobacter pyloriCarrier ProteinsEnzyme InhibitorsEthanolIsoenzymesPrkce protein, ratProtein Kinase CProtein Kinase C-epsilon

Identifiers

PMID10536000
PMCPMC23099
OpenAlexW2043169483

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.