ArticleProceedings of the National Academy of Sciences of the United States of America1999
RAG2 is regulated differentially in B and T cells by elements 5' of the promoter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- The Function of E2A in B-Cell Development.Advances in experimental medicine and biology · 2024Review
- CRISPR-Cas9 engineering of the RAG2 locus via complete coding sequence replacement for therapeutic applications.Nature communications · 2023Article
- Perfluoroalkyl substances (PFASs) decrease the expression of recombination-activating genes (RAG1 and RAG2) in human B lymphoma Namalwa cells.Archives of toxicology · 2022Article
- RAG deficiencies: Recent advances in disease pathogenesis and novel therapeutic approaches.European journal of immunology · 2021Review
- Genome-Wide Analysis IdentifiesFrontiers in cell and developmental biology · 2021Article
- The Interplay Between Chromatin Architecture and Lineage-Specific Transcription Factors and the Regulation ofFrontiers in immunology · 2021Review
- Hierarchical assembly and disassembly of a transcriptionally active RAG locus in CD4The Journal of experimental medicine · 2019Article
- Dual Mechanism ofMolecular and cellular biology · 2017Article
- An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development.The Journal of experimental medicine · 2015Article
- Fidelity of a BAC-EGFP transgene in reporting dynamic expression of IL-7Rα in T cells.Transgenic research · 2012Article
- Mechanisms controlling expression of the RAG locus during lymphocyte development.Current opinion in immunology · 2009Review
- From B cell to plasma cell: regulation of V(D)J recombination and antibody secretion.Immunologic research · 2006Review
- E47 is required for V(D)J recombinase activity in common lymphoid progenitors.The Journal of experimental medicine · 2005Article
- Revision of T cell receptor {alpha} chain genes is required for normal T lymphocyte development.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A role for nuclear factor kappa B/rel transcription factors in the regulation of the recombinase activator genes.Immunity · 2005Article
- B lineage-specific regulation of V(D)J recombinase activity is established in common lymphoid progenitors.The Journal of experimental medicine · 2004Article
- The role of recombination activating gene (RAG) reinduction in thymocyte development in vivo.The Journal of experimental medicine · 2001Article
- Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development.Immunology · 2000Review
- c-Myb binds to a sequence in the proximal region of the RAG-2 promoter and is essential for promoter activity in T-lineage cells.Molecular and cellular biology · 2000Article
- Receptor revision of immunoglobulin heavy chain variable region genes in normal human B lymphocytes.The Journal of experimental medicine · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To study RAG2 gene regulation in vivo, we developed a blastocyst complementation method in which RAG2-deficient embryonic stem cells were transfected with genomic clones containing RAG2 and then assessed for their ability to generate lymphocytes. A RAG2 genomic clone that contained only the RAG2 promoter sequences rescued V(D)J recombination in RAG2-deficient pro-B cell lines, but did not rescue development of RAG2-deficient lymphocytes in vivo. However, inclusion of varying lengths of sequences 5' of the RAG2 promoter generated constructs capable of rescuing only in vivo B cell development, as well as other constructs that rescued both B and T cell development. In particular, the 2-kb 5' region starting just upstream of the RAG2 promoter, as well as the region from 2-7 kb 5', could independently drive B cell development, but not efficient T cell development. Deletion of the 2-kb 5' region from the murine germ line demonstrated that this region was not required for RAG expression sufficient to generate normal B or T cell numbers, implying redundancy among 5' elements. We conclude that RAG2 expression in vivo requires elements beyond the core promoter, that such elements contribute to differential regulation in the B vs. T lineages, and that sequences sufficient to direct B cell expression are located in the promoter-proximal 5' region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.