ArticleProceedings of the National Academy of Sciences of the United States of America1999
Rapid treadmilling of brain microtubules free of microtubule-associated proteins in vitro and its suppression by tau.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Epothilone B stabilizes microtubules via Tau to protect retinal ganglion cells and promote axon regeneration.Cell communication and signaling : CCS · 2026Article
- Self-organization of mortal filaments and its role in bacterial division ring formation.Nature physics · 2024Article
- Tau, microtubule dynamics, and axonal transport: New paradigms for neurodegenerative disease.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Microtubule Dysfunction: A Common Feature of Neurodegenerative Diseases.International journal of molecular sciences · 2020Review
- Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- A computational framework for cortical microtubule dynamics in realistically shaped plant cells.PLoS computational biology · 2018Article
- A conceptual view at microtubule plus end dynamics in neuronal axons.Brain research bulletin · 2016Review
- Tau Structures.Frontiers in aging neuroscience · 2016Review
- Dual-specificity tyrosine phosphorylation-regulated kinase 1A (Dyrk1A) modulates serine/arginine-rich protein 55 (SRp55)-promoted Tau exon 10 inclusion.The Journal of biological chemistry · 2012Article
- The neuroprotective peptide NAP does not directly affect polymerization or dynamics of reconstituted neural microtubules.Journal of Alzheimer's disease : JAD · 2010Article
- Carbendazim inhibits cancer cell proliferation by suppressing microtubule dynamics.The Journal of pharmacology and experimental therapeutics · 2009Article
- Article
- Tau isoform regulation is region- and cell-specific in mouse brain.The Journal of comparative neurology · 2008Article
- Microtubule assembly of isotypically purified tubulin and its mixtures.Biophysical journal · 2008Article
- Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein.Journal of virology · 2006Article
- Modulation of microtubule dynamics by tau in living cells: implications for development and neurodegeneration.Molecular biology of the cell · 2004Article
- Differential regulation of microtubule dynamics by three- and four-repeat tau: implications for the onset of neurodegenerative disease.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Protein kinase MARK/PAR-1 is required for neurite outgrowth and establishment of neuronal polarity.Molecular biology of the cell · 2002Article
- The three modern faces of mercury.Environmental health perspectives · 2002Review
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Authors and funding
3 authors.
Funding
Abstract
We have determined the treadmilling rate of brain microtubules (MTs) free of MT-associated proteins (MAPs) at polymer mass steady state in vitro by using [(3)H]GTP-exchange. We developed buffer conditions that suppressed dynamic instability behavior by approximately 10-fold to minimize the contribution of dynamic instability to total tubulin-GTP exchange. The MTs treadmilled rapidly under the suppressed dynamic instability conditions, at a minimum rate of 0.2 micrometer/min. Thus, rapid treadmilling is an intrinsic property of MAP-free MTs. Further, we show that tau, an axonal stabilizing MAP involved in Alzheimer's disease, strongly suppresses the treadmilling rate. These results indicate that tau's function in axons might involve suppression of axonal MT treadmilling. We describe mathematically how treadmilling and dynamic instability are mechanistically distinct MT behaviors. Finally, we present a model that explains how small changes in the critical tubulin subunit concentration at MT minus ends, caused by intrinsic differences in rate constants or regulatory proteins, could produce large changes in the treadmilling rate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.